skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Parathyroid hormone stimulation of collagenase secretion by isolated bone cells. [Rats]

Abstract

Cells isolated from fetal rat calvaria were cultured in a (/sup 14/C)glycine-labeled collagen gel. This method of culture places the cells in an environment similar to that in the intact tissue and provides a means for assaying the release of collagenase from the cells. Degradation of the collagen matrix begins within the first 3 h of cell culture, the earliest time point at which samples were taken, and continues for at least 12 h. When cells were prepared from calvaria incubated for 30 min with parathyroid hormone (PTH), there was a marked decrease in the collagenase content of the freshly isolated cells, indicating that secretion of collagenase had been enhanced by PTH. Upon subsequent culture of control and PTH-pretreated cells, there was an increase in lysis of collagen gels surrounding the hormone-treated cells within 8 h. The increased collagenolysis was prevented by exposure of the cells to puromycin before and during culture, suggesting that collagenase synthesis also was stimulated by PTH.

Authors:
;
Publication Date:
Research Org.:
Univ. of Rochester, NY
OSTI Identifier:
6296041
Alternate Identifier(s):
OSTI ID: 6296041
Resource Type:
Journal Article
Journal Name:
Endocrinology; (United States)
Additional Journal Information:
Journal Volume: 104:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; COLLAGEN; DECOMPOSITION; ENZYMES; SECRETION; PARATHORMONE; BIOLOGICAL EFFECTS; PUROMYCIN; BONE CELLS; CELL CULTURES; EXPERIMENTAL DATA; FETUSES; GRAPHS; ISOLATED VALUES; LYSIS; RATS; ANIMAL CELLS; ANIMALS; ANTIBIOTICS; CHEMICAL REACTIONS; CONNECTIVE TISSUE CELLS; DATA; DATA FORMS; DRUGS; HORMONES; INFORMATION; MAMMALS; NUMERICAL DATA; ORGANIC COMPOUNDS; PEPTIDE HORMONES; PROTEINS; RODENTS; SCLEROPROTEINS; SOMATIC CELLS; VERTEBRATES 550200* -- Biochemistry

Citation Formats

Puzas, J.E., and Brand, J.S. Parathyroid hormone stimulation of collagenase secretion by isolated bone cells. [Rats]. United States: N. p., 1979. Web.
Puzas, J.E., & Brand, J.S. Parathyroid hormone stimulation of collagenase secretion by isolated bone cells. [Rats]. United States.
Puzas, J.E., and Brand, J.S. Thu . "Parathyroid hormone stimulation of collagenase secretion by isolated bone cells. [Rats]". United States.
@article{osti_6296041,
title = {Parathyroid hormone stimulation of collagenase secretion by isolated bone cells. [Rats]},
author = {Puzas, J.E. and Brand, J.S.},
abstractNote = {Cells isolated from fetal rat calvaria were cultured in a (/sup 14/C)glycine-labeled collagen gel. This method of culture places the cells in an environment similar to that in the intact tissue and provides a means for assaying the release of collagenase from the cells. Degradation of the collagen matrix begins within the first 3 h of cell culture, the earliest time point at which samples were taken, and continues for at least 12 h. When cells were prepared from calvaria incubated for 30 min with parathyroid hormone (PTH), there was a marked decrease in the collagenase content of the freshly isolated cells, indicating that secretion of collagenase had been enhanced by PTH. Upon subsequent culture of control and PTH-pretreated cells, there was an increase in lysis of collagen gels surrounding the hormone-treated cells within 8 h. The increased collagenolysis was prevented by exposure of the cells to puromycin before and during culture, suggesting that collagenase synthesis also was stimulated by PTH.},
doi = {},
journal = {Endocrinology; (United States)},
number = ,
volume = 104:2,
place = {United States},
year = {1979},
month = {2}
}