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Title: Temperature and nucleotide dependence of calcium release by myo-inositol 1,4,5-trisphosphate in cultured vascular smooth muscle cells

Abstract

Inositol 1,4,5-trisphosphate (IP3) rapidly increased UVCaS efflux from a nonmitochondrial organelle in cultured vascular smooth muscle cells that were permeabilized with saponin. A nucleotide, preferably ATP, was essential for IP3-evoked UVCaS release. Two nonhydrolyzable ATP analogues satisfied the nucleotide requirement for IP3-evoked UVCaS release. IP3 strongly stimulated UVCaS efflux at low temperatures (1 to 15 degrees C). Decreasing the temperature from 37 to 4 degrees C inhibited the rate of IP3-stimulated efflux by only about 33%. The failure of such low temperatures to strongly inhibit IP3-induced UVCaS efflux suggests that IP3 activated a CaS channel, rather than a carrier, by a ligand-binding, rather than a metabolic, reaction.

Authors:
; ;
Publication Date:
Research Org.:
Univ. of Alabama, Birmingham
OSTI Identifier:
6292299
Resource Type:
Journal Article
Resource Relation:
Journal Name: J. Biol. Chem.; (United States); Journal Volume: 27
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CALCIUM; MEMBRANE TRANSPORT; ATP; BIOCHEMICAL REACTION KINETICS; CALCIUM 45; CATIONS; CELL CULTURES; INOSITOL; MICE; MUSCLES; NUCLEOTIDES; PERMEABILITY; SAPONINS; STRUCTURE-ACTIVITY RELATIONSHIPS; TEMPERATURE DEPENDENCE; TRACER TECHNIQUES; ALKALINE EARTH ISOTOPES; ALKALINE EARTH METALS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CALCIUM ISOTOPES; CARBOHYDRATES; CHARGED PARTICLES; DAYS LIVING RADIOISOTOPES; ELEMENTS; EVEN-ODD NUCLEI; GLYCOSIDES; INOSITOLS; INTERMEDIATE MASS NUCLEI; IONS; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MAMMALS; METALS; MONOSACCHARIDES; NUCLEI; ORGANIC COMPOUNDS; RADIOISOTOPES; REACTION KINETICS; RODENTS; SACCHARIDES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Smith, J.B., Smith, L., and Higgins, B.L. Temperature and nucleotide dependence of calcium release by myo-inositol 1,4,5-trisphosphate in cultured vascular smooth muscle cells. United States: N. p., 1985. Web.
Smith, J.B., Smith, L., & Higgins, B.L. Temperature and nucleotide dependence of calcium release by myo-inositol 1,4,5-trisphosphate in cultured vascular smooth muscle cells. United States.
Smith, J.B., Smith, L., and Higgins, B.L. Mon . "Temperature and nucleotide dependence of calcium release by myo-inositol 1,4,5-trisphosphate in cultured vascular smooth muscle cells". United States.
@article{osti_6292299,
title = {Temperature and nucleotide dependence of calcium release by myo-inositol 1,4,5-trisphosphate in cultured vascular smooth muscle cells},
author = {Smith, J.B. and Smith, L. and Higgins, B.L.},
abstractNote = {Inositol 1,4,5-trisphosphate (IP3) rapidly increased UVCaS efflux from a nonmitochondrial organelle in cultured vascular smooth muscle cells that were permeabilized with saponin. A nucleotide, preferably ATP, was essential for IP3-evoked UVCaS release. Two nonhydrolyzable ATP analogues satisfied the nucleotide requirement for IP3-evoked UVCaS release. IP3 strongly stimulated UVCaS efflux at low temperatures (1 to 15 degrees C). Decreasing the temperature from 37 to 4 degrees C inhibited the rate of IP3-stimulated efflux by only about 33%. The failure of such low temperatures to strongly inhibit IP3-induced UVCaS efflux suggests that IP3 activated a CaS channel, rather than a carrier, by a ligand-binding, rather than a metabolic, reaction.},
doi = {},
journal = {J. Biol. Chem.; (United States)},
number = ,
volume = 27,
place = {United States},
year = {Mon Nov 25 00:00:00 EST 1985},
month = {Mon Nov 25 00:00:00 EST 1985}
}