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Title: Fetal nicotine exposure produces postnatal up-regulation of adenylate cyclase activity in peripheral tissues

Abstract

Gestational exposure to nicotine has been shown to affect development of noradrenergic activity in both the central and peripheral nervous systems. In the current study, pregnant rats received nicotine infusions of 6 mg/kg/day throughout gestation, administered by osmotic minipump implants. After birth, offspring of the nicotine-infused dams exhibited marked increases in basal adenylate cyclase activity in membranes prepared from kidney and heart, as well as supersensitivity to stimulation by either a {beta}-adrenergic agonist, isoproterenol, or by forskolin. The altered responses were not accompanied by up-regulation of {beta}-adrenergic receptors: in fact, ({sup 125}I)pindolol binding was significantly decreased in the nicotine group. These results indicate that fetal nicotine exposure affects enzymes involved in membrane receptor signal transduction, leading to altered responsiveness independently of changes at the receptor level.

Authors:
; ; ;  [1]
  1. (Duke Univ. Medical Center, Durham, NC (USA))
Publication Date:
OSTI Identifier:
6291816
Resource Type:
Journal Article
Journal Name:
Life Sciences; (USA)
Additional Journal Information:
Journal Volume: 47:17; Journal ID: ISSN 0024-3205
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CYCLASES; ENZYME ACTIVITY; NICOTINE; BIOLOGICAL EFFECTS; BIOCHEMICAL REACTION KINETICS; CELL MEMBRANES; FETUSES; IODINE 125; NERVOUS SYSTEM; PREGNANCY; PRENATAL EXPOSURE; RATS; RECEPTORS; SYMPATHOLYTICS; TRACER TECHNIQUES; ALKALOIDS; AMINES; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; AZINES; AZOLES; BETA DECAY RADIOISOTOPES; CELL CONSTITUENTS; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; HETEROCYCLIC COMPOUNDS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LYASES; MAMMALS; MEMBRANE PROTEINS; MEMBRANES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PARASYMPATHOLYTICS; PARASYMPATHOMIMETICS; PROTEINS; PYRIDINES; PYRROLES; PYRROLIDINES; RADIOISOTOPES; REACTION KINETICS; RODENTS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Slotkin, T.A., Navarro, H.A., McCook, E.C., and Seidler, F.J. Fetal nicotine exposure produces postnatal up-regulation of adenylate cyclase activity in peripheral tissues. United States: N. p., 1990. Web. doi:10.1016/0024-3205(90)90185-T.
Slotkin, T.A., Navarro, H.A., McCook, E.C., & Seidler, F.J. Fetal nicotine exposure produces postnatal up-regulation of adenylate cyclase activity in peripheral tissues. United States. doi:10.1016/0024-3205(90)90185-T.
Slotkin, T.A., Navarro, H.A., McCook, E.C., and Seidler, F.J. Mon . "Fetal nicotine exposure produces postnatal up-regulation of adenylate cyclase activity in peripheral tissues". United States. doi:10.1016/0024-3205(90)90185-T.
@article{osti_6291816,
title = {Fetal nicotine exposure produces postnatal up-regulation of adenylate cyclase activity in peripheral tissues},
author = {Slotkin, T.A. and Navarro, H.A. and McCook, E.C. and Seidler, F.J.},
abstractNote = {Gestational exposure to nicotine has been shown to affect development of noradrenergic activity in both the central and peripheral nervous systems. In the current study, pregnant rats received nicotine infusions of 6 mg/kg/day throughout gestation, administered by osmotic minipump implants. After birth, offspring of the nicotine-infused dams exhibited marked increases in basal adenylate cyclase activity in membranes prepared from kidney and heart, as well as supersensitivity to stimulation by either a {beta}-adrenergic agonist, isoproterenol, or by forskolin. The altered responses were not accompanied by up-regulation of {beta}-adrenergic receptors: in fact, ({sup 125}I)pindolol binding was significantly decreased in the nicotine group. These results indicate that fetal nicotine exposure affects enzymes involved in membrane receptor signal transduction, leading to altered responsiveness independently of changes at the receptor level.},
doi = {10.1016/0024-3205(90)90185-T},
journal = {Life Sciences; (USA)},
issn = {0024-3205},
number = ,
volume = 47:17,
place = {United States},
year = {1990},
month = {1}
}