Isolation and characterization of cDNA clones for carrot extensin and a proline-rich 33-kDa protein
Extensins are hydroxyproline-rich glycoproteins associated with most dicotyledonous plant cell walls. To isolate cDNA clones encoding extensin, the authors started by isolating poly(A) RNA from carrot root tissue, and then translating the RNA in vitro, in the presence of tritiated leucine or proline. A 33-kDa peptide was identified in the translation products as a putative extensin precursor. From a cDNA library constructed with poly(A) RNA from wounded carrots, one cDNA clone (pDC5) was identified that specifically hybridized to poly(A) RNA encoding this 33-kDa peptide. They isolated three cDNA clones (pDC11, pDC12, and pDC16) from another cDNA library using pCD5 as a probe. DNA sequence data, RNA hybridization analysis, and hybrid released in vitro translation indicate that the cDNA clones pDC11 encodes extensin and that cDNA clones pDC12 and pDC16 encode the 33-kDa peptide, which as yet has an unknown identity and function. The assumption that the 33-kDa peptide was an extensin precursor was invalid. RNA hybridization analysis showed that RNA encoded by both clone types is accumulated upon wounding.
- Research Organization:
- Washington Univ., St. Louis, MO
- DOE Contract Number:
- FG02-84ER13255
- OSTI ID:
- 6290620
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 82:13
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLUCOPROTEINS
BIOCHEMISTRY
RECOMBINANT DNA
DNA SEQUENCING
TRITIUM COMPOUNDS
CARROTS
CELL WALL
DNA-CLONING
HYBRIDIZATION
LEUCINE
MESSENGER-RNA
PLANT CELLS
PROLINE
WOUNDS
AMINES
AMINO ACIDS
AZOLES
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMISTRY
CLONING
DNA
FOOD
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INJURIES
LABELLED COMPOUNDS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PROTEINS
PYRROLES
PYRROLIDINES
RNA
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
VEGETABLES
550401* - Genetics- Tracer Techniques
550201 - Biochemistry- Tracer Techniques