Applications of mass spectrometry to DNA fingerprinting and DNA sequencing

Conference · Fri Jan 01 00:00:00 EST 1993

OSTI ID:6288647

Jacobson, K B; Buchanan, M V; Chen, C H; Doktycz, M J; McLuckey, S A ^[1]; Arlinghaus, H F ^[2]

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1. Oak Ridge National Lab., TN (United States)
2. Atom Sciences, Inc., Oak Ridge, TN (United States)

DNA fingerprinting and sequencing rely on polyacrylamide gel electrophoresis to determine the sizes of the DNA fragments. Innovative altematives to polyacrylamide gel electrophoresis are under investigation for characterization of such fingerprinting and sequencing. One method uses stable isotopes of tin and other elements to label the DNAwhereas other procedures do not require labels. The detectors in each case are mass spectrometers that detect either the stable isotopes or the DNA fragments themselves. If successful, these methods will speed up the rate of DNA analysis by one or two orders of magnitude.

• Research Organization: Oak Ridge National Lab., TN (United States); Atom Sciences, Inc., Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: AC05-84OR21400; FG05-90ER81048
• OSTI ID: 6288647
• Report Number(s): CONF-9303192-1; ON: DE93015904
• Resource Relation: Conference: 2. international symposium on the forensic aspects of DNA analysis, Quantico, VA (United States), 29 Mar - 2 Apr 1993
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA SEQUENCING
OLIGONUCLEOTIDES
DERIVATIZATION
MASS SPECTROSCOPY
ELECTROPHORESIS
LASER SPECTROSCOPY
ORGANOMETALLIC COMPOUNDS
RHODAMINES
TIN 116
TIN 118
TIN COMPOUNDS
AMINES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DYES
EVEN-EVEN NUCLEI
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPES
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
REAGENTS
SPECTROSCOPY
STABLE ISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
TIN ISOTOPES
550201* - Biochemistry- Tracer Techniques
550401 - Genetics- Tracer Techniques