Ultraviolet light-induced crosslinking reveals a unique region of local tertiary structure in potato spindle tuber viroid and HeLa 5S RNA
The positions of intramolecular crosslinks induced by irradiation with ultraviolet light were mapped into potato spindle tuber viroid RNA and HeLa 5S rRNA. Crosslinking in each of these molecules occurred at a single major site, which was located by RNA fingerprinting and secondary analysis. Various lines of evidence suggest that these crosslinks identify a previously undescribed element of local tertiary structure common to these two widely divergent RNA molecules: (i) both crosslinks occur in an identical eight-base context, with the sequence 5 GGGAA 3 on one side and the sequence 5 UAC 3 on the other; (ii) both crosslinks connect bases that are not thought to be involved in conventional hydrogen bonding, within regions usually depicted as single-stranded loops flanked by short helical segments; and (iii) both crosslinks connect a purine and a pyrimidine residue, and both may generate the same G-U dimer. Furthermore, it is likely that the crosslinking site is of functional significance because it is located within the most highly conserved region of the viroid sequence and involves bases that are essentially invariant among eukaryotic 5S rRNA molecules.
- Research Organization:
- Rockefeller Univ., New York, NY
- OSTI ID:
- 6287244
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 19
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CROSS-LINKING
RADIOINDUCTION
RNA
BIOLOGICAL RADIATION EFFECTS
HELA CELLS
RIBOSOMES
ULTRAVIOLET RADIATION
VIRUSES
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CHEMICAL REACTIONS
ELECTROMAGNETIC RADIATION
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PARASITES
POLYMERIZATION
RADIATION EFFECTS
RADIATIONS
560121* - Radiation Effects on Cells- External Source- (-1987)