skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: In vitro growth inhibition of mastitis causing bacteria by phenolics and metal chelators

Abstract

Antimicrobial activities of three phenolic compounds and four metal chelators were tested at 0, 250, 500, and 1000 ppm in vitro against four major mastitis-causing bacteria, Streptococcus agalactiae, Staphylococcus aureus, Klebsiella pnuemoniae, and Escherichia coli. Overall, butylated hydroxyanisole and tert-butylhydroquinone showed the greatest antimicrobial activity. These phenolics were bactericidal at 250 to 500 ppm against all four bacteria tested. The butylated hydroxytoluene was bactericidal against the gram-positive bacteria but was ineffective against the coliforms. At 250 ppm, disodium ethylenediaminetetraacetic acid was bactericidal against the gram-positive bacteria but much less effective against the gram-negatives. However, diethylene-triaminepentaacetic acid was more growth inhibitory than ethylenediaminetetraacetic acid against the gram-negative bacteria and especially against Escherichia coli. All other compounds were generally much less effective or ineffective against all four microorganisms. Therefore, butylated hydroxyanisole, butylated hydroxytoluene, tert-butylhydroquinone, ethylenediaminetetraacetic acid, and diethylenetriaminepentaacetic acid may have practical implications in the prevention or treatment of bovine mastitis.

Authors:
; ;
Publication Date:
Research Org.:
Washington State Univ., Pullman
OSTI Identifier:
6285852
Resource Type:
Journal Article
Journal Name:
J. Dairy Sci.; (United States)
Additional Journal Information:
Journal Volume: 68:11
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ANTIOXIDANTS; BIOLOGICAL EFFECTS; BACTERIA; GROWTH; EDTA; CATTLE; COMPARATIVE EVALUATIONS; ESCHERICHIA COLI; IN VITRO; INFECTIOUS DISEASES; INHIBITION; KLEBSIELLA; MAMMARY GLANDS; PHENOLS; STAPHYLOCOCCUS; STREPTOCOCCUS; AMINO ACIDS; ANIMALS; AROMATICS; BODY; CARBOXYLIC ACIDS; CHELATING AGENTS; DISEASES; DOMESTIC ANIMALS; GLANDS; HYDROXY COMPOUNDS; MAMMALS; MICROORGANISMS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; RUMINANTS; VERTEBRATES; 560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)

Citation Formats

Chew, B.P., Tjoelker, L.W., and Tanaka, T.S. In vitro growth inhibition of mastitis causing bacteria by phenolics and metal chelators. United States: N. p., 1985. Web. doi:10.3168/jds.S0022-0302(85)81199-1.
Chew, B.P., Tjoelker, L.W., & Tanaka, T.S. In vitro growth inhibition of mastitis causing bacteria by phenolics and metal chelators. United States. doi:10.3168/jds.S0022-0302(85)81199-1.
Chew, B.P., Tjoelker, L.W., and Tanaka, T.S. Fri . "In vitro growth inhibition of mastitis causing bacteria by phenolics and metal chelators". United States. doi:10.3168/jds.S0022-0302(85)81199-1.
@article{osti_6285852,
title = {In vitro growth inhibition of mastitis causing bacteria by phenolics and metal chelators},
author = {Chew, B.P. and Tjoelker, L.W. and Tanaka, T.S.},
abstractNote = {Antimicrobial activities of three phenolic compounds and four metal chelators were tested at 0, 250, 500, and 1000 ppm in vitro against four major mastitis-causing bacteria, Streptococcus agalactiae, Staphylococcus aureus, Klebsiella pnuemoniae, and Escherichia coli. Overall, butylated hydroxyanisole and tert-butylhydroquinone showed the greatest antimicrobial activity. These phenolics were bactericidal at 250 to 500 ppm against all four bacteria tested. The butylated hydroxytoluene was bactericidal against the gram-positive bacteria but was ineffective against the coliforms. At 250 ppm, disodium ethylenediaminetetraacetic acid was bactericidal against the gram-positive bacteria but much less effective against the gram-negatives. However, diethylene-triaminepentaacetic acid was more growth inhibitory than ethylenediaminetetraacetic acid against the gram-negative bacteria and especially against Escherichia coli. All other compounds were generally much less effective or ineffective against all four microorganisms. Therefore, butylated hydroxyanisole, butylated hydroxytoluene, tert-butylhydroquinone, ethylenediaminetetraacetic acid, and diethylenetriaminepentaacetic acid may have practical implications in the prevention or treatment of bovine mastitis.},
doi = {10.3168/jds.S0022-0302(85)81199-1},
journal = {J. Dairy Sci.; (United States)},
number = ,
volume = 68:11,
place = {United States},
year = {1985},
month = {11}
}