Pregnancy-induced insulin resistance in liver and skeletal muscles of the conscious rabbit
Insulin sensitivity of maternal nonuterine tissues (liver and skeletal muscles) has been investigated in the conscious rabbit during late gestation (24 and 30 days). The specific effect of insulin on glucose production and utilization was evaluated with the hyperinsulinemic euglycemic clamp technique using two types of labelled microspheres (VXCo and TSn). The net balance of glucose across the hindlimb muscles was studied by means of the Fick principle in basal and insulin stimulated conditions (clamp study). The results show that an insulin-resistant state developed between days 24 and 30 of gestation in the rabbit and involves both glucose producing (liver) and utilizing (muscles) tissues. On day 30 of gestation, muscle glucose uptake was not significantly stimulated at a plasma insulin concentration of 700 U/ml determined by radioimmunoassay, whereas it was stimulated by 30-40% in nonpregnant and 24 day pregnant rabbits. At similar plasma insulin concentration, endogenous glucose production was suppressed by 85% in both nonpregnant and 24 day pregnant rabbits, whereas it was decreased by only 30% in 30 day pregnant rabbits. The present data suggest that hindlimb muscles of late pregnant rabbits are able to reduce their insulin-induced glucose utilization. This could contribute to meet the glucose requirements of pregnant uterus in late gestation.
- Research Organization:
- Centre National de la Recherche Scientifique, Meudon-Bellevue, France
- OSTI ID:
- 6283893
- Journal Information:
- Am. J. Physiol.; (United States), Vol. 252:2
- Country of Publication:
- United States
- Language:
- English
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BLOOD FLOW
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PREGNANCY
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MICROSPHERES
MUSCLES
RABBITS
TIN 113
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BETA DECAY RADIOISOTOPES
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ISOTOPE APPLICATIONS
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MAMMALS
MINUTES LIVING RADIOISOTOPES
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550601* - Medicine- Unsealed Radionuclides in Diagnostics