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Title: Prokaryotic adenylate cyclase toxin stimulates anterior pituitary cells in culture

Abstract

Bordetella pertussis synthesis a variety of virulence factors including a calmodulin-dependent adenylate cyclase (AC) toxin. Treatment of anterior pituitary cells with this AC toxin resulted in an increase in cellular cAMP levels that was associated with accelerated exocytosis of growth hormone (GH), prolactin, adrenocorticotropic hormone (ACTH), and luteinizing hormone (LH). The kinetics of release of these hormones, however, were markedly different; GH and prolactin were rapidly released, while LH and ACTH secretion was more gradually elevated. Neither dopamine agonists nor somatostatin changes the ability of AC toxin to generate cAMP (up to 2 h). Low concentrations of AC toxin amplified the secretory response to hypophysiotrophic hormones. The authors conclude that bacterial AC toxin can rapidly elevate cAMP levels in anterior pituitary cells and that it is the response that explains the subsequent acceleration of hormone release.

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Univ. of Virginia School of Medicine, Charlottesville
OSTI Identifier:
6283311
Resource Type:
Journal Article
Journal Name:
Am. J. Physiol.; (United States)
Additional Journal Information:
Journal Volume: 251:2
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ACTH; RADIOIMMUNOASSAY; SECRETION; CYCLASES; BIOLOGICAL EFFECTS; LH; LTH; STH; AMP; ANIMAL CELLS; BACTERIA; DOPAMINE; PITUITARY GLAND; RATS; TOXINS; AMINES; ANIMALS; ANTIGENS; AROMATICS; AUTONOMIC NERVOUS SYSTEM AGENTS; BODY; CARDIOTONICS; CARDIOVASCULAR AGENTS; DRUGS; ENDOCRINE GLANDS; ENZYMES; GLANDS; GONADOTROPINS; HORMONES; HYDROXY COMPOUNDS; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; LYASES; MAMMALS; MATERIALS; MICROORGANISMS; NEUROREGULATORS; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; PHENOLS; PITUITARY HORMONES; POLYPHENOLS; RADIOASSAY; RADIOIMMUNOLOGY; RODENTS; SYMPATHOMIMETICS; TOXIC MATERIALS; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Cronin, M.J., Evans, W.S., Rogol, A.D., Weiss, A.A., Thorner, M.O., Orth, D.N., Nicholson, W.E., Yasumoto, T., and Hewlett, E.L. Prokaryotic adenylate cyclase toxin stimulates anterior pituitary cells in culture. United States: N. p., 1986. Web.
Cronin, M.J., Evans, W.S., Rogol, A.D., Weiss, A.A., Thorner, M.O., Orth, D.N., Nicholson, W.E., Yasumoto, T., & Hewlett, E.L. Prokaryotic adenylate cyclase toxin stimulates anterior pituitary cells in culture. United States.
Cronin, M.J., Evans, W.S., Rogol, A.D., Weiss, A.A., Thorner, M.O., Orth, D.N., Nicholson, W.E., Yasumoto, T., and Hewlett, E.L. Fri . "Prokaryotic adenylate cyclase toxin stimulates anterior pituitary cells in culture". United States.
@article{osti_6283311,
title = {Prokaryotic adenylate cyclase toxin stimulates anterior pituitary cells in culture},
author = {Cronin, M.J. and Evans, W.S. and Rogol, A.D. and Weiss, A.A. and Thorner, M.O. and Orth, D.N. and Nicholson, W.E. and Yasumoto, T. and Hewlett, E.L.},
abstractNote = {Bordetella pertussis synthesis a variety of virulence factors including a calmodulin-dependent adenylate cyclase (AC) toxin. Treatment of anterior pituitary cells with this AC toxin resulted in an increase in cellular cAMP levels that was associated with accelerated exocytosis of growth hormone (GH), prolactin, adrenocorticotropic hormone (ACTH), and luteinizing hormone (LH). The kinetics of release of these hormones, however, were markedly different; GH and prolactin were rapidly released, while LH and ACTH secretion was more gradually elevated. Neither dopamine agonists nor somatostatin changes the ability of AC toxin to generate cAMP (up to 2 h). Low concentrations of AC toxin amplified the secretory response to hypophysiotrophic hormones. The authors conclude that bacterial AC toxin can rapidly elevate cAMP levels in anterior pituitary cells and that it is the response that explains the subsequent acceleration of hormone release.},
doi = {},
journal = {Am. J. Physiol.; (United States)},
number = ,
volume = 251:2,
place = {United States},
year = {1986},
month = {8}
}