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Title: Transdermal delivery of a melanotropic peptide hormone analogue

Abstract

We previously reported that topical application of (Nl3/sup 4/,D-Phe/sup 7/)alpha-MSH, a superpotent analogue of alpha-melanocyte stimulating hormone, to mice induces a darkening of follicular melanocytes throughout the skin. We now report that the melanotropin analogue can be delivered across mouse but not rat skin in an in vitro model system. Passage of the analogue from the topically applied vehicle (polyethylene glycol) across the skin into a subcutaneous receiving vessel was demonstrated by both bioassay as well as by radioimmunoassay. The bioassay data demonstrate that percutaneous absorption of the melanotropin did not result in loss of biological activity of the peptide. The differential penetration of the peptide across rodent skin reveals that one cannot predict percutaneous absorption of a substance across the stratum corneum from studies on a single species. The present results are the first to demonstrate, by direct quantitative measurements, that a bioactive peptide can be delivered across the vertebrate integument in vitro. These studies point out the potential of a topically applied melanotropin for tanning of the skin and possibly for treatment of certain hypopigmentary disorders.

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Univ. of Arizona, Tucson (USA)
OSTI Identifier:
6281841
Resource Type:
Journal Article
Resource Relation:
Journal Name: Life Sci.; (United States); Journal Volume: 43:14
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HORMONES; SKIN ABSORPTION; SKIN DISEASES; CHEMOTHERAPY; BIOLOGICAL MODELS; GENETIC VARIABILITY; IN VITRO; MICE; PEPTIDES; RADIOIMMUNOASSAY; RATS; ABSORPTION; ANIMALS; BIOLOGICAL VARIABILITY; DISEASES; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; MAMMALS; ORGANIC COMPOUNDS; PROTEINS; RADIOASSAY; RADIOIMMUNOLOGY; RODENTS; THERAPY; TRACER TECHNIQUES; UPTAKE; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques; 550901 - Pathology- Tracer Techniques

Citation Formats

Dawson, B.V., Hadley, M.E., Kreutzfeld, K., Dorr, R.T., Hruby, V.J., Al-Obeidi, F., and Don, S. Transdermal delivery of a melanotropic peptide hormone analogue. United States: N. p., 1988. Web. doi:10.1016/0024-3205(88)90469-9.
Dawson, B.V., Hadley, M.E., Kreutzfeld, K., Dorr, R.T., Hruby, V.J., Al-Obeidi, F., & Don, S. Transdermal delivery of a melanotropic peptide hormone analogue. United States. doi:10.1016/0024-3205(88)90469-9.
Dawson, B.V., Hadley, M.E., Kreutzfeld, K., Dorr, R.T., Hruby, V.J., Al-Obeidi, F., and Don, S. 1988. "Transdermal delivery of a melanotropic peptide hormone analogue". United States. doi:10.1016/0024-3205(88)90469-9.
@article{osti_6281841,
title = {Transdermal delivery of a melanotropic peptide hormone analogue},
author = {Dawson, B.V. and Hadley, M.E. and Kreutzfeld, K. and Dorr, R.T. and Hruby, V.J. and Al-Obeidi, F. and Don, S.},
abstractNote = {We previously reported that topical application of (Nl3/sup 4/,D-Phe/sup 7/)alpha-MSH, a superpotent analogue of alpha-melanocyte stimulating hormone, to mice induces a darkening of follicular melanocytes throughout the skin. We now report that the melanotropin analogue can be delivered across mouse but not rat skin in an in vitro model system. Passage of the analogue from the topically applied vehicle (polyethylene glycol) across the skin into a subcutaneous receiving vessel was demonstrated by both bioassay as well as by radioimmunoassay. The bioassay data demonstrate that percutaneous absorption of the melanotropin did not result in loss of biological activity of the peptide. The differential penetration of the peptide across rodent skin reveals that one cannot predict percutaneous absorption of a substance across the stratum corneum from studies on a single species. The present results are the first to demonstrate, by direct quantitative measurements, that a bioactive peptide can be delivered across the vertebrate integument in vitro. These studies point out the potential of a topically applied melanotropin for tanning of the skin and possibly for treatment of certain hypopigmentary disorders.},
doi = {10.1016/0024-3205(88)90469-9},
journal = {Life Sci.; (United States)},
number = ,
volume = 43:14,
place = {United States},
year = 1988,
month = 1
}
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