Kinetics of chlorinated hydrocarbon degradation by suspended cultures of methane-oxidizing bacteria

Journal Article · · Research Journal of the Water Pollution Control Federation; (USA)

OSTI ID:6271909

Strand, S E; Bjelland, M D; Stensel, H D

The kinetics of methane utilization and the biodegradation of trichloroethylene (TCE) and 1,1,1-trichloroethane (TCA) by a mixed, methanotrophic bacterial culture were studied in a closed-system reactor. Methane oxidation followed Michaelis-Menten kinetics. TCE and TCA degradation followed first-order kinetics with rate constants of 3.7 {times} 10{sup {minus}4} and 8.8 {times} 10{sup {minus}5} L/mg VSS {center dot} h, respectively, for concentrations less than 3,000 {mu}g/L. Oxidative activity of the methanotrophic culture ceased at a dissolved TCE concentration of 7,770 {mu}g/L. TCA, but not TCE, biodegradation was inhibited by dissolved methane concentrations in excess of 0.25 mg/L. In the absence of methane, the culture continued to degrade TCE and TCA, but degradation ceased after 104 hours. Lower biodegradation rates were observed when treating a mixture of TCE and TCA.

• OSTI ID: 6271909
• Journal Information: Research Journal of the Water Pollution Control Federation; (USA), Vol. 62:2; ISSN 1047-7624
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
CHLORINATED ALIPHATIC HYDROCARBONS
BIODEGRADATION
METHANOTROPHIC BACTERIA
METABOLISM
METHANE
OXIDATION
ALKANES
BACTERIA
CHEMICAL REACTIONS
DECOMPOSITION
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROCARBONS
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
098000* - Biomass Fuels- Waste Management- (1990-)