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Title: Herpes simplex virus in postradiation cervical smears. A morphologic and immunocytochemical study

Abstract

From January 1987 to August 1988, cytomorphologic criteria of both herpes simplex virus (HSV) and radiation effects were observed in Papanicolaou smears from 3 of 1,340 patients who had received radiotherapy for squamous cell carcinoma of the cervix. Avidin-biotin immunoperoxidase staining, using a rabbit IgG polyclonal HSV antibody, confirmed the presence of HSV antigen in those three postradiation smears. Both multinucleated molded cells and epithelial cells that lacked cytopathic effects were positive for HSV. Three other postradiation smears from these cases were similarly positive for HSV antigen; the one preradiation smear was negative. In situ hybridization and immunoperoxidase studies on sections from the preradiation biopsies were negative: severely altered neoplastic cells showed no reactivity. The absence of HSV markers in the preradiation specimens suggests that the HSV infections were secondary to the radiotherapy; further studies are needed to prove this association and to assess the possible mechanisms. These cases clearly indicate that the overlapping features of radiation and viral effects (such as multinucleation) may be present simultaneously.

Authors:
; ; ; ;  [1]
  1. (Adolfo Lutz Institute, Sao Paulo State Public Health Service (Brazil))
Publication Date:
OSTI Identifier:
6270927
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Cytologica; (USA); Journal Volume: 34:5
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CARCINOMAS; RADIOTHERAPY; SIDE EFFECTS; UTERUS; VIRUSES; BIOLOGICAL RADIATION EFFECTS; FEMALE GENITALS; HERPES SIMPLEX; PATIENTS; BIOLOGICAL EFFECTS; BODY; DISEASES; INFECTIOUS DISEASES; MEDICINE; MICROORGANISMS; NEOPLASMS; NUCLEAR MEDICINE; ORGANS; PARASITES; RADIATION EFFECTS; RADIOLOGY; SKIN DISEASES; THERAPY; VIRAL DISEASES; 560151* - Radiation Effects on Animals- Man; 560130 - Radiation Effects on Microorganisms

Citation Formats

Longatto Filho, A., Maeda, M.Y., Oyafuso, M.S., Kanamura, C.T., and Alves, V.A. Herpes simplex virus in postradiation cervical smears. A morphologic and immunocytochemical study. United States: N. p., 1990. Web.
Longatto Filho, A., Maeda, M.Y., Oyafuso, M.S., Kanamura, C.T., & Alves, V.A. Herpes simplex virus in postradiation cervical smears. A morphologic and immunocytochemical study. United States.
Longatto Filho, A., Maeda, M.Y., Oyafuso, M.S., Kanamura, C.T., and Alves, V.A. Sat . "Herpes simplex virus in postradiation cervical smears. A morphologic and immunocytochemical study". United States.
@article{osti_6270927,
title = {Herpes simplex virus in postradiation cervical smears. A morphologic and immunocytochemical study},
author = {Longatto Filho, A. and Maeda, M.Y. and Oyafuso, M.S. and Kanamura, C.T. and Alves, V.A.},
abstractNote = {From January 1987 to August 1988, cytomorphologic criteria of both herpes simplex virus (HSV) and radiation effects were observed in Papanicolaou smears from 3 of 1,340 patients who had received radiotherapy for squamous cell carcinoma of the cervix. Avidin-biotin immunoperoxidase staining, using a rabbit IgG polyclonal HSV antibody, confirmed the presence of HSV antigen in those three postradiation smears. Both multinucleated molded cells and epithelial cells that lacked cytopathic effects were positive for HSV. Three other postradiation smears from these cases were similarly positive for HSV antigen; the one preradiation smear was negative. In situ hybridization and immunoperoxidase studies on sections from the preradiation biopsies were negative: severely altered neoplastic cells showed no reactivity. The absence of HSV markers in the preradiation specimens suggests that the HSV infections were secondary to the radiotherapy; further studies are needed to prove this association and to assess the possible mechanisms. These cases clearly indicate that the overlapping features of radiation and viral effects (such as multinucleation) may be present simultaneously.},
doi = {},
journal = {Acta Cytologica; (USA)},
number = ,
volume = 34:5,
place = {United States},
year = {Sat Sep 01 00:00:00 EDT 1990},
month = {Sat Sep 01 00:00:00 EDT 1990}
}