Membrane fractions active in poliovirus RNA replication contain VPg precursor polypeptides

Journal Article · Sat Jan 01 00:00:00 EST 1983 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(83)90316-1· OSTI ID:6267082

Takegami, T; Semler, B L; Anderson, C W; Wimmer, E

The poliovirus specific polypeptide P3-9 is of special interest for studies of viral RNA replication because it contains a hydrophobic region and, separated by only seven amino acids from that region, the amino acid sequence of the genome-linked protein VPg. Membraneous complexes of poliovirus-infected HeLa cells that contain poliovirus RNA replicating proteins have been analyzed for the presence of P3-9 by immunoprecipitation. Incubation of a membrane fraction rich in P3-9 with proteinase leaves the C-terminal 69 amino acids of P3-9 intact, an observation suggesting that this portion is protected by its association with the cellular membrane. These studies have also revealed two hitherto undescribed viral polypeptides consisting of amino acid sequences of the P2 andf P3 regions of the polyprotein. Sequence analysis by stepwise Edman degradation show that these proteins are 3b/9 (M/sub r/77,000) and X/9 (M/sub r/50,000). 3b/9 and X/9 are membrane bound and are turned over rapidly and may be direct precursors to proteins P2-X and P3-9 of the RNA replication complex. P2-X, a polypeptide void of hydrophobic amino acid sequences but also found associated with membranes, is rapidly degraded when the membraneous complex is treated with trypsin. It is speculated that P2-X is associated with membranes by its affinity to the N-terminus of P3-9.

• Research Organization: State Univ. of New York, Stony Brook
• OSTI ID: 6267082
• Journal Information: Virology; (United States), Vol. 128
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL MEMBRANES
STRUCTURAL CHEMICAL ANALYSIS
POLIO VIRUS
NUCLEIC ACID REPLICATION
AMINO ACID SEQUENCE
ANTIBODIES
BIODEGRADATION
ENZYMES
HELA CELLS
MOLECULAR BIOLOGY
POLYPEPTIDES
RADIOIMMUNOASSAY
RNA
TRYPSIN
CELL CONSTITUENTS
CHEMICAL REACTIONS
DECOMPOSITION
HYDROLASES
ISOTOPE APPLICATIONS
MEMBRANES
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PEPTIDE HYDROLASES
PEPTIDES
PROTEINS
RADIOASSAY
SERINE PROTEINASES
TRACER TECHNIQUES
VIRUSES
550201* - Biochemistry- Tracer Techniques