Inhibitory effects of titanium(III) citrate on enumeration of bacteria from rumen contents

Journal Article · Wed Aug 01 00:00:00 EDT 1984 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6264217

Wachenheim, D E; Hespell, R B

Titanium citrate (TC) or L-cysteine-sodium sulfide was added as a reducing agent to buffers and agar media used for enumeration of bacteria from rumen contents of high-forage-fed steers. Approximately equal colony counts were found on TC and L-cysteine-sodium sulfide-reduced media with rumen contents taken 8 h postfeeding, when active bacterial growth was occurring. The colony counts on TC medium were only 56% of those with L-cysteine-sodium sulfide medium with rumen contents taken 1 h prefeeding when bacterial growth was minimal. When colonies from L-cysteine-sodium sulfide medium were transferred to TC medium and vice versa, almost all colonies grew. The data indicate that TC can be inhibitory to bacteria upon their initial isolation from natural habitats, particularly when growth rates are low in these habitats.

• Research Organization: Univ. of Illinois, Urbana
• OSTI ID: 6264217
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 48:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
SENSITIVITY
GROWTH
INHIBITION
TITANIUM COMPOUNDS
TOXICITY
CATTLE
COMPARATIVE EVALUATIONS
CULTURE MEDIA
REDUCING AGENTS
STOMACH
ANIMALS
BODY
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
GASTROINTESTINAL TRACT
MAMMALS
MICROORGANISMS
ORGANS
RUMINANTS
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)