skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Cell surface glycopeptides from human intestinal epithelial cell lines derived from normal colon and colon adenocarcinomas

Abstract

The cell surface glycopeptides from an epithelial cell line (CCL 239) derived from normal human colon were compared with those from three cell lines (HCT-8R, HCT-15, and CaCo-2) derived independently from human colonic adenocarcinomas. Cells were incubated with D-(2-TH)mannose or L-(5,6-TH)fucose for 24 h and treated with trypsin to release cell surface components which were then digested exhaustively with Pronase and fractionated on Bio-Gel P-6 before and after treatment with endo-beta-N-acetylglucosaminidase H. The most noticeable difference between the labeled glycopeptides from the tumor and CCL 239 cells was the presence in the former of an endo-beta-N-acetylglucosaminidase H-resistant high molecular weight glycopeptide fraction which was eluted in the void volume of Bio-Gel P-6. This fraction was obtained with both labeled mannose and fucose as precursors. However, acid hydrolysis of this fraction obtained after incubation with (2-TH)mannose revealed that as much as 60-90% of the radioactivity was recovered as fucose. Analysis of the total glycopeptides (cell surface and cell pellet) obtained after incubation with (2-TH)mannose showed that from 40-45% of the radioactivity in the tumor cells and less than 10% of the radioactivity in the CCL 239 cells was recovered as fucose. After incubation of the HCT-8R cells with D-(1,6-TH)glucosamine and L-(1-more » UC)fucose, strong acid hydrolysis of the labeled glycopeptide fraction excluded from Bio-Gel P-6 produced TH-labeled N-acetylglucosamine and N-acetylgalactosamine.« less

Authors:
;
Publication Date:
Research Org.:
McGill Univ., Montreal, Quebec
OSTI Identifier:
6258910
Resource Type:
Journal Article
Journal Name:
Cancer Res.; (United States)
Additional Journal Information:
Journal Name: Cancer Res.; (United States)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CARBON 14 COMPOUNDS; TRACER TECHNIQUES; GLUCOPROTEINS; BIOCHEMISTRY; TRITIUM COMPOUNDS; ACID HYDROLYSIS; ANIMAL CELLS; CARCINOMAS; GALACTOSE; INTESTINES; MANNOSE; TUMOR CELLS; ALDEHYDES; BODY; CARBOHYDRATES; CHEMICAL REACTIONS; CHEMISTRY; DECOMPOSITION; DIGESTIVE SYSTEM; DISEASES; GASTROINTESTINAL TRACT; HEXOSES; HYDROLYSIS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LYSIS; MONOSACCHARIDES; NEOPLASMS; ORGANIC COMPOUNDS; ORGANS; PROTEINS; SACCHARIDES; SOLVOLYSIS; 550301* - Cytology- Tracer Techniques

Citation Formats

Youakim, A, and Herscovics, A. Cell surface glycopeptides from human intestinal epithelial cell lines derived from normal colon and colon adenocarcinomas. United States: N. p., 1985. Web.
Youakim, A, & Herscovics, A. Cell surface glycopeptides from human intestinal epithelial cell lines derived from normal colon and colon adenocarcinomas. United States.
Youakim, A, and Herscovics, A. Fri . "Cell surface glycopeptides from human intestinal epithelial cell lines derived from normal colon and colon adenocarcinomas". United States.
@article{osti_6258910,
title = {Cell surface glycopeptides from human intestinal epithelial cell lines derived from normal colon and colon adenocarcinomas},
author = {Youakim, A and Herscovics, A},
abstractNote = {The cell surface glycopeptides from an epithelial cell line (CCL 239) derived from normal human colon were compared with those from three cell lines (HCT-8R, HCT-15, and CaCo-2) derived independently from human colonic adenocarcinomas. Cells were incubated with D-(2-TH)mannose or L-(5,6-TH)fucose for 24 h and treated with trypsin to release cell surface components which were then digested exhaustively with Pronase and fractionated on Bio-Gel P-6 before and after treatment with endo-beta-N-acetylglucosaminidase H. The most noticeable difference between the labeled glycopeptides from the tumor and CCL 239 cells was the presence in the former of an endo-beta-N-acetylglucosaminidase H-resistant high molecular weight glycopeptide fraction which was eluted in the void volume of Bio-Gel P-6. This fraction was obtained with both labeled mannose and fucose as precursors. However, acid hydrolysis of this fraction obtained after incubation with (2-TH)mannose revealed that as much as 60-90% of the radioactivity was recovered as fucose. Analysis of the total glycopeptides (cell surface and cell pellet) obtained after incubation with (2-TH)mannose showed that from 40-45% of the radioactivity in the tumor cells and less than 10% of the radioactivity in the CCL 239 cells was recovered as fucose. After incubation of the HCT-8R cells with D-(1,6-TH)glucosamine and L-(1- UC)fucose, strong acid hydrolysis of the labeled glycopeptide fraction excluded from Bio-Gel P-6 produced TH-labeled N-acetylglucosamine and N-acetylgalactosamine.},
doi = {},
url = {https://www.osti.gov/biblio/6258910}, journal = {Cancer Res.; (United States)},
number = ,
volume = ,
place = {United States},
year = {1985},
month = {11}
}