Protein quality and quantity and insulin control of mammary gland glucose utilization during lactation
Virgin Sprague-Dawley rats were bred, and fed laboratory stock (STOCK), 13% casein plus methionine, 13% wheat gluten, or 5% casein plus methionine through gestation and 4 days of lactation. Diets were switched at parturition to determine the effects of dietary protein quality and quantity fed during gestation and/or lactation on insulin stimulation of mammary glucose utilization. On day 20 of gestation (20G) and day 4 of lactation (4L) the right inguinal-abdominal mammary glands were removed, and acini and tissue slices were incubated in Krebs buffer with or without insulin containing (U-/sup 14/C)-glucose and 5mM glucose for 1 hour at 37/degrees/C. Glucose incorporation into CO/sub 2/, lipid and lactose was determined. Glucose incorporation into CO/sub 2/ and lipid, but not lactose was stimulated by insulin in mammary slices. Diet effects on glucose utilization in acini were confirmed in slices for basal and insulin stimulated levels. Treatment affected the absolute increase of insulin stimulation. Regression analysis significantly correlated pup weight gain with total glucose utilization. Poor dietary protein quality and quantity fed during gestation impaired both overall response of mammary glucose utilization to insulin stimulation, and mammary development during pregnancy. Improving protein value at parturition did not overcome those deficits by 4L.
- Research Organization:
- Colorado State Univ., Fort Collins (USA)
- OSTI ID:
- 6256636
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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GLUCOSE
METABOLISM
INSULIN
BIOCHEMICAL REACTION KINETICS
PROTEINS
INGESTION
CARBON 14 COMPOUNDS
CARBON DIOXIDE
DIET
LACTATION
LIPIDS
MAMMARY GLANDS
METHIONINE
PREGNANCY
RATS
REGRESSION ANALYSIS
TRACER TECHNIQUES
ALDEHYDES
AMINO ACIDS
ANIMALS
BODY
CARBOHYDRATES
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
DRUGS
GLANDS
HEXOSES
HORMONES
INTAKE
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
MAMMALS
MATHEMATICS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PEPTIDE HORMONES
REACTION KINETICS
RODENTS
SACCHARIDES
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550501* - Metabolism- Tracer Techniques