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Title: New class of leukemogenic ecotropic recombinant murine leukemia virus isolated from radiation-induced thymomas of C57BL/6 mice

Abstract

We previously reported the establishment of several lymphoid cell lines from X-ray-induced thymomas of C57BL/Ka mice, and all, except one, produce retroviruses. Biological characterization of five of these new primary radiation leukemia viruses (RadLVs) indicated that they had a B-tropic, fibrotropic, and ecotropic host range and were leukemogenic when reinjected into C57BL/Ka newborn mice. The leukemogenic potential of one isolate (G/sub 6/T/sub 2/) was further assessed and shown to be retained after prolonged passaging on fibroblasts in vitro. Restriction endonuclease analysis of the DNA of four of our new RadLV isolates (G/sub 6/T/sub 2/, Ti-7, Ti-8, and Ti-9) revealed that G/sub 6/T/sub 2/ and Ti-7 murine leukemia virus (MuLV) genomes had identical restriction maps, whereas Ti-8 and Ti-9 genomes were different from each other and from the G/sub 6/T/sub 2/ and Ti-7 genomes. The physical maps of these genomes were similar to that of known ecotropic MuLV genomes (including the C57BL/Ka endogenous ecotropic MuLV) within their long terminal repeats, env, the right portion of pol, and the left portion of gag. However, a region covering the end of gag and the beginning of pol was different and showed several similarities with xenotropic MuLV genomes of BALB/c, AKR, and C58 micemore » previously mapped. Our results suggest that these primary RadLV genomes are recombinants between the parental ecotropic MuLV genome and a nonecotropic (xenotropic) sequence. To further study the leukemic potential of these RadLVs, the genome of one of them (G/sub 6/T/sub 2/) was cloned in Charon 21A as an infectious molecule.« less

Authors:
 [1]; ; ; ;
  1. Institut de Recherches Cliniques de Montreal, Canada
Publication Date:
OSTI Identifier:
6253478
Resource Type:
Journal Article
Journal Name:
J. Virol.; (United States)
Additional Journal Information:
Journal Volume: 45:2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; LEUKEMIA VIRUSES; GENE RECOMBINATION; NEOPLASMS; RADIOINDUCTION; X RADIATION; BIOLOGICAL RADIATION EFFECTS; GENETIC VARIABILITY; LEUKEMOGENESIS; MICE; THYMUS; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL VARIABILITY; BODY; CARCINOGENESIS; DISEASES; ELECTROMAGNETIC RADIATION; IONIZING RADIATIONS; LYMPHATIC SYSTEM; MAMMALS; MICROORGANISMS; ONCOGENIC VIRUSES; ORGANS; PARASITES; PATHOGENESIS; RADIATION EFFECTS; RADIATIONS; RODENTS; VERTEBRATES; VIRUSES; 560152* - Radiation Effects on Animals- Animals; 550400 - Genetics

Citation Formats

Rassart, E, Sankar-Mistry, P, Lemay, G, DesGroseillers, L, and Jalicoeur, P. New class of leukemogenic ecotropic recombinant murine leukemia virus isolated from radiation-induced thymomas of C57BL/6 mice. United States: N. p., 1983. Web.
Rassart, E, Sankar-Mistry, P, Lemay, G, DesGroseillers, L, & Jalicoeur, P. New class of leukemogenic ecotropic recombinant murine leukemia virus isolated from radiation-induced thymomas of C57BL/6 mice. United States.
Rassart, E, Sankar-Mistry, P, Lemay, G, DesGroseillers, L, and Jalicoeur, P. Tue . "New class of leukemogenic ecotropic recombinant murine leukemia virus isolated from radiation-induced thymomas of C57BL/6 mice". United States.
@article{osti_6253478,
title = {New class of leukemogenic ecotropic recombinant murine leukemia virus isolated from radiation-induced thymomas of C57BL/6 mice},
author = {Rassart, E and Sankar-Mistry, P and Lemay, G and DesGroseillers, L and Jalicoeur, P},
abstractNote = {We previously reported the establishment of several lymphoid cell lines from X-ray-induced thymomas of C57BL/Ka mice, and all, except one, produce retroviruses. Biological characterization of five of these new primary radiation leukemia viruses (RadLVs) indicated that they had a B-tropic, fibrotropic, and ecotropic host range and were leukemogenic when reinjected into C57BL/Ka newborn mice. The leukemogenic potential of one isolate (G/sub 6/T/sub 2/) was further assessed and shown to be retained after prolonged passaging on fibroblasts in vitro. Restriction endonuclease analysis of the DNA of four of our new RadLV isolates (G/sub 6/T/sub 2/, Ti-7, Ti-8, and Ti-9) revealed that G/sub 6/T/sub 2/ and Ti-7 murine leukemia virus (MuLV) genomes had identical restriction maps, whereas Ti-8 and Ti-9 genomes were different from each other and from the G/sub 6/T/sub 2/ and Ti-7 genomes. The physical maps of these genomes were similar to that of known ecotropic MuLV genomes (including the C57BL/Ka endogenous ecotropic MuLV) within their long terminal repeats, env, the right portion of pol, and the left portion of gag. However, a region covering the end of gag and the beginning of pol was different and showed several similarities with xenotropic MuLV genomes of BALB/c, AKR, and C58 mice previously mapped. Our results suggest that these primary RadLV genomes are recombinants between the parental ecotropic MuLV genome and a nonecotropic (xenotropic) sequence. To further study the leukemic potential of these RadLVs, the genome of one of them (G/sub 6/T/sub 2/) was cloned in Charon 21A as an infectious molecule.},
doi = {},
journal = {J. Virol.; (United States)},
number = ,
volume = 45:2,
place = {United States},
year = {1983},
month = {2}
}