skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: H[sub 2] photoproduction by batch culture of Anabaena variabilis ATCC 29413 and its mutant PK84 in a photobioreactor

Abstract

Hydrogen production by Anabaena variabilis ATCC 29413 and of its mutant PK84, grown in batch cultures, was studied in a photobioreactor. The highest volumetric H[sub 2] production rates of native and mutant strains were found in cultures grown at gradually increased irradiation. The native strain evolved H[sub 2] only under an argon atmosphere with the actual rate as high as the potential rate. In this case 61% of oxygenic photosynthesis was used for H[sub 2]production. In contrast the mutant PK84 produced H[sub 2] during growth under CO[sub 2]-enriched air. Under these conditions at the maximum rate of H[sub 2] production, 13% of oxygenic photosynthesis was used for H[sub 2] production and the actual H[sub 2] production was only 22% of the potential. Under an atmosphere of 98% argon + 2% CO[sub 2] actual H[sub 2] by the mutant was strictly light-dependent with saturation at about 300[mu]E m[sup [minus]2]s[sup [minus]1]. However, the rate of photosynthesis was not saturated at this irradiation. At limiting light intensities 33--58% of photosynthesis was used for J[sub 2] production. Hydrogen evolution by PK84 under air + 2% CO[sub 2] was also stimulated by light; but was not saturated at 332 [mu]E m[sup [minus]2] s[sup [minus]1] andmore » did not cease completely in darkness. The rate of oxygen photoevolution was also not saturated. A mechanism for increasing cyanobacterial hydrogen production is proposed.« less

Authors:
;  [1]; ;  [2]
  1. (Kings Coll. London (United Kingdom) Institute of Basic Biological Problems RAS, Moscow (Russian Federation))
  2. (Kings Coll. London (United Kingdom))
Publication Date:
OSTI Identifier:
6252832
Resource Type:
Journal Article
Journal Name:
Biotechnology and Bioengineering
Additional Journal Information:
Journal Volume: 64:6; Journal ID: ISSN 0006-3592
Country of Publication:
United States
Language:
English
Subject:
14 SOLAR ENERGY; 08 HYDROGEN; BIOREACTORS; CYANOBACTERIA; ENZYME ACTIVITY; HYDROGEN PRODUCTION; MUTANTS; NITROGENASE; PHOTOSYNTHESIS; CHEMICAL REACTIONS; ENZYMES; MICROORGANISMS; NITRO-GROUP DEHYDROGENASES; ORGANIC COMPOUNDS; OXIDOREDUCTASES; PHOTOCHEMICAL REACTIONS; PROTEINS; SYNTHESIS; 140505* - Solar Energy Conversion- Photochemical, Photobiological, & Thermochemical Conversion- (1980-); 080106 - Hydrogen- Production- Biosynthesis & Photochemical Processes

Citation Formats

Tsygankov, A.A., Borodin, V.B., Rao, K.K., and Hall, D.O. H[sub 2] photoproduction by batch culture of Anabaena variabilis ATCC 29413 and its mutant PK84 in a photobioreactor. United States: N. p., 1999. Web. doi:10.1002/(SICI)1097-0290(19990920)64:6<709::AID-BIT10>3.0.CO;2-C.
Tsygankov, A.A., Borodin, V.B., Rao, K.K., & Hall, D.O. H[sub 2] photoproduction by batch culture of Anabaena variabilis ATCC 29413 and its mutant PK84 in a photobioreactor. United States. doi:10.1002/(SICI)1097-0290(19990920)64:6<709::AID-BIT10>3.0.CO;2-C.
Tsygankov, A.A., Borodin, V.B., Rao, K.K., and Hall, D.O. Mon . "H[sub 2] photoproduction by batch culture of Anabaena variabilis ATCC 29413 and its mutant PK84 in a photobioreactor". United States. doi:10.1002/(SICI)1097-0290(19990920)64:6<709::AID-BIT10>3.0.CO;2-C.
@article{osti_6252832,
title = {H[sub 2] photoproduction by batch culture of Anabaena variabilis ATCC 29413 and its mutant PK84 in a photobioreactor},
author = {Tsygankov, A.A. and Borodin, V.B. and Rao, K.K. and Hall, D.O.},
abstractNote = {Hydrogen production by Anabaena variabilis ATCC 29413 and of its mutant PK84, grown in batch cultures, was studied in a photobioreactor. The highest volumetric H[sub 2] production rates of native and mutant strains were found in cultures grown at gradually increased irradiation. The native strain evolved H[sub 2] only under an argon atmosphere with the actual rate as high as the potential rate. In this case 61% of oxygenic photosynthesis was used for H[sub 2]production. In contrast the mutant PK84 produced H[sub 2] during growth under CO[sub 2]-enriched air. Under these conditions at the maximum rate of H[sub 2] production, 13% of oxygenic photosynthesis was used for H[sub 2] production and the actual H[sub 2] production was only 22% of the potential. Under an atmosphere of 98% argon + 2% CO[sub 2] actual H[sub 2] by the mutant was strictly light-dependent with saturation at about 300[mu]E m[sup [minus]2]s[sup [minus]1]. However, the rate of photosynthesis was not saturated at this irradiation. At limiting light intensities 33--58% of photosynthesis was used for J[sub 2] production. Hydrogen evolution by PK84 under air + 2% CO[sub 2] was also stimulated by light; but was not saturated at 332 [mu]E m[sup [minus]2] s[sup [minus]1] and did not cease completely in darkness. The rate of oxygen photoevolution was also not saturated. A mechanism for increasing cyanobacterial hydrogen production is proposed.},
doi = {10.1002/(SICI)1097-0290(19990920)64:6<709::AID-BIT10>3.0.CO;2-C},
journal = {Biotechnology and Bioengineering},
issn = {0006-3592},
number = ,
volume = 64:6,
place = {United States},
year = {1999},
month = {9}
}