H[sub 2] photoproduction by batch culture of Anabaena variabilis ATCC 29413 and its mutant PK84 in a photobioreactor

Journal Article · Mon Sep 20 00:00:00 EDT 1999 · Biotechnology and Bioengineering

DOI:https://doi.org/10.1002/(SICI)1097-0290(19990920)64:6<709::AID-BIT10>3.0.CO;2-C· OSTI ID:6252832

Tsygankov, A A; Borodin, V B ^[1]; Rao, K K; Hall, D O ^[2]

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1. Kings Coll. London (United Kingdom) Institute of Basic Biological Problems RAS, Moscow (Russian Federation)
2. Kings Coll. London (United Kingdom)

Hydrogen production by Anabaena variabilis ATCC 29413 and of its mutant PK84, grown in batch cultures, was studied in a photobioreactor. The highest volumetric H[sub 2] production rates of native and mutant strains were found in cultures grown at gradually increased irradiation. The native strain evolved H[sub 2] only under an argon atmosphere with the actual rate as high as the potential rate. In this case 61% of oxygenic photosynthesis was used for H[sub 2]production. In contrast the mutant PK84 produced H[sub 2] during growth under CO[sub 2]-enriched air. Under these conditions at the maximum rate of H[sub 2] production, 13% of oxygenic photosynthesis was used for H[sub 2] production and the actual H[sub 2] production was only 22% of the potential. Under an atmosphere of 98% argon + 2% CO[sub 2] actual H[sub 2] by the mutant was strictly light-dependent with saturation at about 300[mu]E m[sup [minus]2]s[sup [minus]1]. However, the rate of photosynthesis was not saturated at this irradiation. At limiting light intensities 33--58% of photosynthesis was used for J[sub 2] production. Hydrogen evolution by PK84 under air + 2% CO[sub 2] was also stimulated by light; but was not saturated at 332 [mu]E m[sup [minus]2] s[sup [minus]1] and did not cease completely in darkness. The rate of oxygen photoevolution was also not saturated. A mechanism for increasing cyanobacterial hydrogen production is proposed.

• OSTI ID: 6252832
• Journal Information: Biotechnology and Bioengineering, Vol. 64:6; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

14 SOLAR ENERGY
08 HYDROGEN
BIOREACTORS
CYANOBACTERIA
ENZYME ACTIVITY
HYDROGEN PRODUCTION
MUTANTS
NITROGENASE
PHOTOSYNTHESIS
CHEMICAL REACTIONS
ENZYMES
MICROORGANISMS
NITRO-GROUP DEHYDROGENASES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PHOTOCHEMICAL REACTIONS
PROTEINS
SYNTHESIS
140505* - Solar Energy Conversion- Photochemical
Photobiological
&amp; Thermochemical Conversion- (1980-)
080106 - Hydrogen- Production- Biosynthesis &amp; Photochemical Processes