Prescreening for environmental teratogens using cultured mesenchymal cells from the human embryonic palate

Journal Article · Fri Jan 01 00:00:00 EST 1982 · Teratog., Carcinog., Mutagen.; (United States)

DOI:https://doi.org/10.1002/1520-6866(1990)2:3/4<313::AID-TCM1770020312>3.0.CO;2-C· OSTI ID:6252679

Pratt, R M; Grove, R I; Willis, W D

Mesenchymal cells from the prefusion human embryonic palate have been established in culture and can be grown in either a serum-free hormone-supplemented medium or a serum-containing medium. The growth of these cells is quite rapid in culture and inhibited in a dose-dependent manner by most teratogens thus far tested, such as dexamethasone. These cells are highly sensitive to a variety of DNA synthetic and mitotic inhibitors. The responses of these cells are complementary to the ovarian tumor cell attachment assay of Braun et al (1, and in this volume). When used in conjunction with the tumor cells, the overall reliability is greater than 90% with only one false-negative, allopurinol.

• Research Organization: Experimental Teratogenesis Section, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences-NIH, Research Triangle Park, North Carolina
• OSTI ID: 6252679
• Journal Information: Teratog., Carcinog., Mutagen.; (United States), Vol. 2:3-4
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DEXAMETHASONE
TERATOGEN SCREENING
SOMATIC CELLS
GROWTH
SENSITIVITY
CULTURE MEDIA
DNA
DOSE-RESPONSE RELATIONSHIPS
EMBRYOS
ORAL CAVITY
TERATOGENESIS
TERATOGENS
TISSUE CULTURES
ADRENAL HORMONES
ANIMAL CELLS
CORTICOSTEROIDS
DIGESTIVE SYSTEM
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
KETONES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PREGNANES
SCREENING
STEROID HORMONES
STEROIDS
560301* - Chemicals Metabolism &amp; Toxicology- Cells- (-1987)