Cellular progesterone receptor phosphorylation in response to ligands activating protein kinases
Progesterone receptors were immunoprecipitated with monoclonal antibodies KD68 from lysates of human breast carcinoma T47D cells labelled to steady state specific activity with /sup 32/Pi. The 120 kDa /sup 32/P-labelled progesterone receptor band was resolved by polyacrylamide gel electrophoresis and identified by autoradiography. Phosphoamino acid analysis revealed serine phosphorylation, but no threonine or tyrosine phosphorylation. Treatment of the /sup 32/Pi-labelled cells with EGF, TPA or dibutyryl cAMP had no significant quantitative effect on progesterone receptor phosphorylation, though the EGF receptor and the cAMP-dependent protein kinases have been reported to catalyze phosphorylation of purified avian progesterone receptor preparations in cell free systems. Progesterone receptor phosphorylation on serine residues was increased by 2-fold in cells treated with 10 nM progesterone; EGF had no effect on progesterone-mediated progesterone receptor phosphorylation.
- Research Organization:
- Univ. of California, Los Angeles
- OSTI ID:
- 6245973
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 146:3
- Country of Publication:
- United States
- Language:
- English
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PHOSPHOTRANSFERASES
ENZYME ACTIVITY
PROGESTERONE
RECEPTORS
PHOSPHORYLATION
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
ELECTROPHORESIS
GROWTH FACTORS
IMMUNOASSAY
LIGANDS
PHOSPHATES
PHOSPHORUS 32
RESPONSE MODIFYING FACTORS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ENZYMES
HORMONES
ISOTOPES
KETONES
KINETICS
LIGHT NUCLEI
MEMBRANE PROTEINS
MITOGENS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PREGNANES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
STEROID HORMONES
STEROIDS
TRANSFERASES
550201* - Biochemistry- Tracer Techniques