New analytical techniques for mycotoxins in complex organic matrices
Air samples are collected for analysis from the Ames Solid Waste Recovery System. The high level of airborne fungi within the processing area is of concern due to the possible presence of toxic mycotoxins, and carcinogenic fungal metabolites. An analytical method has been developed to determine the concentration of aflatoxins B1, B2, G1, and G2 in the air of the plant which produces Refuse Derived Fuel (RDF). After extraction with methanol, some components in the matrix are precipitated by dissolving the samples in 30% acetonitrile/chloroform. An aliquot of this solution is injected onto a Styragel column where the sample components undergo simultaneous size exclusion and reverse phase partitioning. The Styragel column appears to have a useable lifetime of more than six months. After elution from Styragel, the sample is diverted to a second column containing Florisil which has been modified with oxalic acid and deactivated with water. Aflatoxins are eluted with 5% water/acetone. After removal of this solvent, the sample is dissolved in 150 ..mu..L of a spotting solvent and the entire sample applied to a thin layer chromatography (TLC) plate using a unique sample applicator developed here. The aflatoxins on the TLC plate are analyzed by laser fluorescence. A detection limit of 10 pg is possible for aflatoxin standards using a nitrogen laser as the excitation source. Sample concentrations are determined by comparing with an internal standard, a specially synthesized aflatoxin derivative. In two separate RDF samples, aflatoxin B1 was found at levels of 6.5 and 17.0 ppB. In a separate study, the spore pigment in Aspergillus flavus was isolated. The mass spectrum indicates a molecular weight in excess of 700. Only aliphatic hydrocarbons have been identified in the mass spectrum of products from a permanganate oxidation.
- DOE Contract Number:
- W-7405-ENG-82
- OSTI ID:
- 6235776
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES
AFLATOXIN
QUANTITATIVE CHEMICAL ANALYSIS
FUNGI
REFUSE DERIVED FUELS
AIR POLLUTION
ASPERGILLUS
INDUSTRIAL PLANTS
SOLVENT EXTRACTION
CHEMICAL ANALYSIS
EXTRACTION
FUELS
PLANTS
POLLUTION
SEPARATION PROCESSES
400105* - Separation Procedures
500200 - Environment
Atmospheric- Chemicals Monitoring & Transport- (-1989)
550700 - Microbiology