Radioimmunoassay of conjugated cholic acid, chenodeoxycholic acid, and deoxycholic acid from human serum, with use of /sup 125/I-labeled ligands
We describe a method for radioimmunoassay of conjugated cholic acid, chenodeoxycholic acid, and deoxycholic acid in serum. In the method, /sup 125/I-labeled bile acid conjugates are used as the tracers along with antibodies raised against individual bile acid-bovine serum albumin conjugates. Antibody-bound and free bile acids were separated by polyethylene glycol precipitation (final concentration, 125 g/L). The lowest measurable amounts of the bile acids, expressed as pmol/tube, were: cholic acid conjugates, 2; chenodeoxycholic acid conjugates, 0.5; and deoxycholic acid conjugates, 2. Analytical recovery of bile acids added to bile acid-free serum ranged from 85 to 110%; intra-assay and inter-assay CVs ranged from 8.3 to 5.3% and from 5.3 to 12.2%, respectively. Concentrations (mean +- SD) of the bile acid conjugates in serum from apparently healthy women and men (in ..mu..mol/L) were: cholic acid conjugates, 0.43 +- 0.17 (n=126); chenodeoxycholic acid conjugates, 0.47 +- 0.23 (n=111); and deoxycholic acid conjugates, 0.33 +- 0.11 (n=96). The values for primary bile acids were greatly increased in patients with various hepatobiliary diseases.
- Research Organization:
- Univ. of Oulu, Finland
- OSTI ID:
- 6228238
- Journal Information:
- Clin. Chem.; (United States), Vol. 25:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHOLIC ACID
RADIOIMMUNOASSAY
ANTIBODIES
BILE ACIDS
BLOOD SERUM
CARBOXYLIC ACIDS
EXPERIMENTAL DATA
GRAPHS
IODINE 125
ISOLATED VALUES
LIGANDS
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
DATA
DATA FORMS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HYDROXY COMPOUNDS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIOASSAY
RADIOISOTOPES
STEROIDS
STEROLS
550601* - Medicine- Unsealed Radionuclides in Diagnostics