Serologic test-systems development: immunoassays for antibiotics. Progress report, May 16, 1980-September 30, 1981
Progress on development of immunoassays for the antibiotics gentamicin, tetracycline, and tylosin is discussed. The development of the gentamicin assay was completed and the assay was transferred to the Beltsville Laboratories of the US Department of Agriculture (USDA) Food Safety and Quality Service (FSQS). The sensitivity (1 ppB) is 50-fold greater than we have previously reported, and is satisfactory for the current needs. At year's end, work was still in progress on both the tetracycline and tylosin assays. Tetracycline presents a more difficult problem of chemistry than we had anticipated. Immunoassay reagents synthesized by diazonium coupling were only weakly immunoreactive; analysis suggests that this coupling procedure damages the tetracycline structure. Several tetracycline derivatives that offer promising alternative coupling procedures were synthesized. Tylosin was successfully coupled to peroxidase. With this conjugate and antiserum, obtained from R. Mageau of the USDA, some immune-specific binding was demonstrated. Several problems with the tylosin assay remain to be resolved.
- Research Organization:
- Los Alamos National Lab., NM (USA)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 6225986
- Report Number(s):
- LA-9692-PR; ON: DE83013322
- Country of Publication:
- United States
- Language:
- English
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