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Title: Improved method for detection of starch hydrolysis

Abstract

A new starch hydrolysis detection method which does not rely on iodine staining or the use of color-complexed starch is described. A linear relationship was obtained with agar-starch plates when net clearing zones around colonies of yeasts were plotted against enzyme levels (semilogarithm scale) produced by the same yeast strains in liquid medium. A similar relationship between starch clearing zones and alpha-amylase levels from three different sources was observed. These observations suggest that the method is useful in mutant isolations, strain improvement programs, and the prediction of alpha-amylase activities in culture filtrates or column effluents. (Refs. 18).

Authors:
; ; ;
Publication Date:
Research Org.:
Agricultural Microbiology Section, Dairy and Food Science Dept, Univ of Saskatchewan, Saskatoon, Canada S7N OWO
OSTI Identifier:
6220653
Alternate Identifier(s):
OSTI ID: 6220653
Resource Type:
Journal Article
Resource Relation:
Journal Name: Appl. Environ. Microbiol.; (United States); Journal Volume: 44:3
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; STARCH; HYDROLYSIS; AMYLASE; ENZYME ACTIVITY; ENZYMES; INDICATORS; MUTANTS; YEASTS; CARBOHYDRATES; CHEMICAL REACTIONS; DECOMPOSITION; FUNGI; GLYCOSYL HYDROLASES; HYDROLASES; LYSIS; MICROORGANISMS; O-GLYCOSYL HYDROLASES; ORGANIC COMPOUNDS; PLANTS; POLYSACCHARIDES; REAGENTS; SACCHARIDES; SOLVOLYSIS 140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989); 090222 -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)

Citation Formats

Ohawale, M.R., Wilson, J.J., Khachatourians, G.G., and Ingledew, W.M.. Improved method for detection of starch hydrolysis. United States: N. p., 1982. Web.
Ohawale, M.R., Wilson, J.J., Khachatourians, G.G., & Ingledew, W.M.. Improved method for detection of starch hydrolysis. United States.
Ohawale, M.R., Wilson, J.J., Khachatourians, G.G., and Ingledew, W.M.. Wed . "Improved method for detection of starch hydrolysis". United States. doi:.
@article{osti_6220653,
title = {Improved method for detection of starch hydrolysis},
author = {Ohawale, M.R. and Wilson, J.J. and Khachatourians, G.G. and Ingledew, W.M.},
abstractNote = {A new starch hydrolysis detection method which does not rely on iodine staining or the use of color-complexed starch is described. A linear relationship was obtained with agar-starch plates when net clearing zones around colonies of yeasts were plotted against enzyme levels (semilogarithm scale) produced by the same yeast strains in liquid medium. A similar relationship between starch clearing zones and alpha-amylase levels from three different sources was observed. These observations suggest that the method is useful in mutant isolations, strain improvement programs, and the prediction of alpha-amylase activities in culture filtrates or column effluents. (Refs. 18).},
doi = {},
journal = {Appl. Environ. Microbiol.; (United States)},
number = ,
volume = 44:3,
place = {United States},
year = {Wed Sep 01 00:00:00 EDT 1982},
month = {Wed Sep 01 00:00:00 EDT 1982}
}