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Title: In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy

Abstract

An intracellular coenzyme has been observed by carbon-13 nuclear magnetic resonance spectroscopy. The pyridine nucleotides in Escherichia coli were specifically labeled with carbon-13 from the biosynthetic precursor, nicotinic acid. The intracellular redox status and metabolic transformations of the pyridine nucleotides were examined under a variety of conditions. A highly reduced nicotinamide adenine dinucleotide pool was observed under anaerobic conditions only in cells that were cultured aerobically on glycerol.

Authors:
; ;
Publication Date:
Research Org.:
Los Alamos National Lab., NM
OSTI Identifier:
6220339
Resource Type:
Journal Article
Journal Name:
Science (Washington, D.C.); (United States)
Additional Journal Information:
Journal Volume: 222
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CARBON 13; NUCLEAR MAGNETIC RESONANCE; ESCHERICHIA COLI; METABOLISM; BIOCHEMICAL REACTION KINETICS; ENZYME ACTIVITY; NICOTINIC ACID; LABELLED COMPOUNDS; NUCLEOTIDES; LABELLING; PYRIDINE; REDOX REACTIONS; IN VIVO; AZINES; BACTERIA; CARBON ISOTOPES; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; EVEN-ODD NUCLEI; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; ISOTOPES; KINETICS; LIGHT NUCLEI; MAGNETIC RESONANCE; MICROORGANISMS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIDINES; REACTION KINETICS; RESONANCE; STABLE ISOTOPES; VITAMIN B GROUP; VITAMINS; 560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)

Citation Formats

Unkefer, C J, Blazer, R M, and London, R E. In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy. United States: N. p., 1983. Web. doi:10.1126/science.6353573.
Unkefer, C J, Blazer, R M, & London, R E. In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy. United States. https://doi.org/10.1126/science.6353573
Unkefer, C J, Blazer, R M, and London, R E. Fri . "In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy". United States. https://doi.org/10.1126/science.6353573.
@article{osti_6220339,
title = {In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy},
author = {Unkefer, C J and Blazer, R M and London, R E},
abstractNote = {An intracellular coenzyme has been observed by carbon-13 nuclear magnetic resonance spectroscopy. The pyridine nucleotides in Escherichia coli were specifically labeled with carbon-13 from the biosynthetic precursor, nicotinic acid. The intracellular redox status and metabolic transformations of the pyridine nucleotides were examined under a variety of conditions. A highly reduced nicotinamide adenine dinucleotide pool was observed under anaerobic conditions only in cells that were cultured aerobically on glycerol.},
doi = {10.1126/science.6353573},
url = {https://www.osti.gov/biblio/6220339}, journal = {Science (Washington, D.C.); (United States)},
number = ,
volume = 222,
place = {United States},
year = {1983},
month = {10}
}