Genetic transfer in Desulfovibrio desulfuricans

Journal Article · Tue Dec 01 00:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.24.9128· OSTI ID:6218044

Rapp, B J; Wall, J D

An apparently defective bacteriophage capable of mediating transduction has been identified in culture filtrates of Desulfovibrio desulfuricans (American Type Culture Collection 27774). Phage-mediated intraspecies transfer of antibiotic resistance markers occurs with a frequency of 10/sup -4/ to 10/sup -6/ per recipient cell. The vector contains linear fragments of double-strained DNA of about 13.5 kilobase pairs, which appear to be random pieces of bacterial DNA. As yet, neither induction nor plaque formation has been observed. To the authors' knowledge, a system of genetic exchange has not been described before for a member of the sulfate-reducing bacteria.

• Research Organization: Univ. of Missouri, Columbia (USA)
• DOE Contract Number: FG02-87ER13713
• OSTI ID: 6218044
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:24
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DESULFOVIBRIO
GENE RECOMBINATION
AUTORADIOGRAPHY
BACTERIOPHAGES
DNA
ELECTROPHORESIS
PHOSPHORUS 32
TRANSMISSION ELECTRON MICROSCOPY
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON MICROSCOPY
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
MICROSCOPY
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SULFATE-REDUCING BACTERIA
VIRUSES
550401* - Genetics- Tracer Techniques