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Title: Self-regulating dynamic control of electroosmotic flow in capillary electrophoresis

Abstract

Capillary electrophoresis (CE), primarily because of the impressive separation power and the compatibility with large biomolecules, has established itself as an important separation tool over the past several years. One of the major barriers limiting the broad, routine application of the technique is the validation of the reliability of migration times and the quantitative accuracy when dealing with real samples. In particular, sample matrices can alter the nature of the surface of the inner wall of the capillary, leading to irreproducible results. Even when the temperature is constant and when adsorption of the analytes onto the capillary wall can be neglected, which is the case of pure zone electrophoresis (CZE), the [zeta] potential of the wall can still be altered by the injected ions. In this report, the authors demonstrate the utility of a concept for maintaining a constant electroosmotic flow rate for sample ions in CZE, independent of the changing sample matrix composition. Such an interactive control of electroosmotic flow has the advantage that the instantaneous flow rate need not be monitored. Once the stationary front is established, no further external intervention is needed. 23 refs., 2 tabs.

Authors:
;  [1]
  1. Ames Lab., IA (United States)
Publication Date:
OSTI Identifier:
6217327
DOE Contract Number:  
W-7405-ENG-82
Resource Type:
Journal Article
Journal Name:
Analytical Chemistry (Washington); (United States)
Additional Journal Information:
Journal Volume: 65:5; Journal ID: ISSN 0003-2700
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; ELECTROPHORESIS; CONTROL; AMMONIUM COMPOUNDS; BROMIDES; CAPILLARIES; CAPILLARY FLOW; EXPERIMENTAL DATA; OSMOSIS; SURFACTANTS; BLOOD VESSELS; BODY; BROMINE COMPOUNDS; CARDIOVASCULAR SYSTEM; DATA; DIFFUSION; FLUID FLOW; HALIDES; HALOGEN COMPOUNDS; INFORMATION; NUMERICAL DATA; ORGANS; 400105* - Separation Procedures; 400400 - Electrochemistry

Citation Formats

Chang, Huan-Tsung, and Yeung, E S. Self-regulating dynamic control of electroosmotic flow in capillary electrophoresis. United States: N. p., 1993. Web. doi:10.1021/ac00053a028.
Chang, Huan-Tsung, & Yeung, E S. Self-regulating dynamic control of electroosmotic flow in capillary electrophoresis. United States. doi:10.1021/ac00053a028.
Chang, Huan-Tsung, and Yeung, E S. Mon . "Self-regulating dynamic control of electroosmotic flow in capillary electrophoresis". United States. doi:10.1021/ac00053a028.
@article{osti_6217327,
title = {Self-regulating dynamic control of electroosmotic flow in capillary electrophoresis},
author = {Chang, Huan-Tsung and Yeung, E S},
abstractNote = {Capillary electrophoresis (CE), primarily because of the impressive separation power and the compatibility with large biomolecules, has established itself as an important separation tool over the past several years. One of the major barriers limiting the broad, routine application of the technique is the validation of the reliability of migration times and the quantitative accuracy when dealing with real samples. In particular, sample matrices can alter the nature of the surface of the inner wall of the capillary, leading to irreproducible results. Even when the temperature is constant and when adsorption of the analytes onto the capillary wall can be neglected, which is the case of pure zone electrophoresis (CZE), the [zeta] potential of the wall can still be altered by the injected ions. In this report, the authors demonstrate the utility of a concept for maintaining a constant electroosmotic flow rate for sample ions in CZE, independent of the changing sample matrix composition. Such an interactive control of electroosmotic flow has the advantage that the instantaneous flow rate need not be monitored. Once the stationary front is established, no further external intervention is needed. 23 refs., 2 tabs.},
doi = {10.1021/ac00053a028},
journal = {Analytical Chemistry (Washington); (United States)},
issn = {0003-2700},
number = ,
volume = 65:5,
place = {United States},
year = {1993},
month = {3}
}