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Title: Study of the repair of potentially lethal and sublethal radiation damage in Chinese hamster cells exposed to extremely hypo- or hypertonic NaCl solutions. [X rays]

Abstract

The effect of 0.05 and 1.5 M NaCl solutions on the repair of potentially lethal damage (PLD) and sublethal damage (SLD) in irradiated Chinese hamster ovary (CHO) cells has been examined. A 20-min exposure to the 0.05 or 1.5 M salt solutions which was terminated immediately before irradiation had no effect on the repair of PLD. However, salt treatment during or after irradiation caused an inhibition of repair of PLD. The possibility that 0.05 or 1.5 M treatment irreversibly affected repair enzymes could be ruled out. Exposure of cells to 0.05 or 1.5 M NaCl solutions before or during the first x-ray dose of a split-dose regimen decreased the ability of the cell to repair SLD. The recovery observed in the experiments was characterized by a change in D/sub 0/ of the survival curves and was related to the loss of interaction of radiation-induced lesions and salt effects as the incubation time between the second x-ray dose and salt treatment increased. However, a partial return of the shoulder of the survival curve when salt treatment preceded split-dose irradiation indicated that some repair of SLD does occur. In summary, salt treatment prior to irradiation does not inhibit repair of either SLDmore » or PLD, whereas, salt treatment during or after irradiation inhibits repair of both SLD and PLD.« less

Authors:
;
Publication Date:
Research Org.:
Colorado State Univ., Fort Collins
OSTI Identifier:
6210429
Resource Type:
Journal Article
Journal Name:
Radiat. Res.; (United States)
Additional Journal Information:
Journal Volume: 77:2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ANIMAL CELLS; SURVIVAL TIME; BIOLOGICAL REPAIR; INHIBITION; DNA; RADIATION INJURIES; RADIOSENSITIVITY EFFECTS; TIME DEPENDENCE; SODIUM CHLORIDES; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; ACUTE IRRADIATION; CELL CULTURES; EXPERIMENTAL DATA; FRACTIONATED IRRADIATION; HAMSTERS; HYPERTONIC SOLUTIONS; ISOLATED VALUES; LETHAL IRRADIATION; SUBLETHAL IRRADIATION; SURVIVAL CURVES; X RADIATION; ACUTE EXPOSURE; ALKALI METAL COMPOUNDS; ANIMALS; BIOLOGICAL RECOVERY; CHLORIDES; CHLORINE COMPOUNDS; DATA; DATA FORMS; DISPERSIONS; ELECTROMAGNETIC RADIATION; HALIDES; HALOGEN COMPOUNDS; INFORMATION; INJURIES; IONIZING RADIATIONS; IRRADIATION; MAMMALS; MIXTURES; NUCLEIC ACIDS; NUMERICAL DATA; ORGANIC COMPOUNDS; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; RODENTS; SODIUM COMPOUNDS; SOLUTIONS; VERTEBRATES; 560121* - Radiation Effects on Cells- External Source- (-1987)

Citation Formats

Raaphorst, G.P., and Dewey, W.C. Study of the repair of potentially lethal and sublethal radiation damage in Chinese hamster cells exposed to extremely hypo- or hypertonic NaCl solutions. [X rays]. United States: N. p., 1979. Web. doi:10.2307/3575144.
Raaphorst, G.P., & Dewey, W.C. Study of the repair of potentially lethal and sublethal radiation damage in Chinese hamster cells exposed to extremely hypo- or hypertonic NaCl solutions. [X rays]. United States. doi:10.2307/3575144.
Raaphorst, G.P., and Dewey, W.C. Thu . "Study of the repair of potentially lethal and sublethal radiation damage in Chinese hamster cells exposed to extremely hypo- or hypertonic NaCl solutions. [X rays]". United States. doi:10.2307/3575144.
@article{osti_6210429,
title = {Study of the repair of potentially lethal and sublethal radiation damage in Chinese hamster cells exposed to extremely hypo- or hypertonic NaCl solutions. [X rays]},
author = {Raaphorst, G.P. and Dewey, W.C.},
abstractNote = {The effect of 0.05 and 1.5 M NaCl solutions on the repair of potentially lethal damage (PLD) and sublethal damage (SLD) in irradiated Chinese hamster ovary (CHO) cells has been examined. A 20-min exposure to the 0.05 or 1.5 M salt solutions which was terminated immediately before irradiation had no effect on the repair of PLD. However, salt treatment during or after irradiation caused an inhibition of repair of PLD. The possibility that 0.05 or 1.5 M treatment irreversibly affected repair enzymes could be ruled out. Exposure of cells to 0.05 or 1.5 M NaCl solutions before or during the first x-ray dose of a split-dose regimen decreased the ability of the cell to repair SLD. The recovery observed in the experiments was characterized by a change in D/sub 0/ of the survival curves and was related to the loss of interaction of radiation-induced lesions and salt effects as the incubation time between the second x-ray dose and salt treatment increased. However, a partial return of the shoulder of the survival curve when salt treatment preceded split-dose irradiation indicated that some repair of SLD does occur. In summary, salt treatment prior to irradiation does not inhibit repair of either SLD or PLD, whereas, salt treatment during or after irradiation inhibits repair of both SLD and PLD.},
doi = {10.2307/3575144},
journal = {Radiat. Res.; (United States)},
number = ,
volume = 77:2,
place = {United States},
year = {1979},
month = {2}
}