Complement complex C5b-8 induces PGI/sub 2/ formation in culture endothelial cells

Journal Article · Wed Jul 01 00:00:00 EDT 1987 · Am. J. Physiol.; (United States)

OSTI ID:6207984

Suttorp, N; Seeger, W; Zinsky, S; Bhakdi, S

The effects of the terminal complement sequence on prostacyclin (PGI/sub 2/) generation in antibody-sensitized pulmonary arterial endothelial cells were examined. Whereas C5b-7 complement complexes induced no PGI/sub 2/ formation, addition of purified complement component C8 resulted in a time- and dose-dependent burst of PGI/sub 2/ release in the absence of overt cell damage. Formation of the complete terminal complement complex C5b-9 enhanced PGI/sub 2/ release but was accompanied by cytolysis. Extracellular Ca/sup 2 +/ was required for C5b-8-dependent PGI/sub 2/ formation. Three different blockers of physiological calcium channels failed to suppress the observed stimulatory effect. In contrast, W7 (N-(6-amino-hexyl)-5-chloro-1-napththalene sulfonamide) and trifluoperazine, inhibitors of calmodulin activity, all reduced the C5b-8-dependent PGI/sub 2/ generation. None of the inhibitors used impaired Ca/sup 2 +/ flux into the cells. One minute after addition of C8 to endothelial cells carrying C5b-7 complexes, a six- to seven-fold enhanced passive influx of /sup 45/Ca/sup 2 +/ into the cells was noted. An enhanced passive influx was also observed for /sup 51/CrO/sub 4//sup 2 -/, (/sup H/) aminobutyric acid, and (/sup 3/H) sucrose, but not for (/sup 3/H)inulin and (/sup 3/H)dextran. These data together suggest that complement C5b-8 complexes may serve as Ca/sup 2 +/bypass gates in endothelial cells, the ensuring influx of Ca/sup 2 +/ leading to subsequent activation of the arachiodonic acid pathway.

• Research Organization: Justus Liebig Univ. of Giessen (Germany, F.R.)
• OSTI ID: 6207984
• Journal Information: Am. J. Physiol.; (United States), Vol. 253:1
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
CALCIUM 45
UPTAKE
CHROMIUM 51
COMPLEMENT
BIOCHEMISTRY
PROSTAGLANDINS
RADIOIMMUNOASSAY
TRITIUM COMPOUNDS
ANTIBODIES
BLOOD SERUM
CHROMATES
DEXTRAN
ENDOTHELIUM
ENZYME INHIBITORS
INULIN
PHYSIOLOGY
RABBITS
SACCHAROSE
TIME DEPENDENCE
ALKALINE EARTH ISOTOPES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD SUBSTITUTES
BODY
CALCIUM ISOTOPES
CARBOHYDRATES
CHEMISTRY
CHROMIUM COMPOUNDS
CHROMIUM ISOTOPES
DAYS LIVING RADIOISOTOPES
DISACCHARIDES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HEMATOLOGIC AGENTS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLYSACCHARIDES
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SACCHARIDES
TISSUES
TRACER TECHNIQUES
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques