Molecular cloning of the. cap alpha. subunit of human and guinea pig leukocyte adhesion glycoprotein Mo1: Chromosomal localization and homology to the. cap alpha. subunits of integrins
The cell surface-glycoprotein Mo1 is a member of the family of leukocyte cell adhesion molecules (Leu-CAMs) that includes lymphocyte function-associated antigen 1 (LFA-1) and p150,95. Each Leu-CAM is a heterodimer with a distinct ..cap alpha.. subunit noncovalently associated with a common ..beta.. subunit. The authors describe the isolation and analysis of two partial cDNA clones encoding the ..cap alpha.. subunit of the Leu-CAM Mo1 in humans and guinea pigs. A monoclonal antibody directed against an epitope in the carboxyl-terminal portion of the guinea pig ..cap alpha.. chain was used for immunoscreening a lambdagt11 expression library. The sequence of a 378-base-pair insert from one immunoreactive clone revealed a single continuous open reading frame encoding 126 amino acids including a 26-amino acid tryptic peptide isolated from the purified guinea pig ..cap alpha.. subunit. A cDNA clone of identical size was isolated from a human monocyte/lymphocyte cDNA library by using the guinea pig clone as a probe. The human clone also encoded a 126-amino acid peptide including the sequence of an additional tryptic peptide present in purified human Mo1..cap alpha.. chain. Southern analysis of DNA from hamster-human hybrids localized the human Mo1..cap alpha.. chain to chromosome 16, which has been shown to contain the gene for the ..cap alpha.. chain of lymphocyte function-associated antigen 1. These data suggest that the ..cap alpha.. subunits of Leu-CAMs evolved by gene duplication from a common ancestral gene and strengthen the hypothesis that the ..cap alpha.. subunits of these heterodimeric cell adhesion molecules on myeloid and lymphoid cells, platelets, and fibroblasts are evolutionary related.
- Research Organization:
- Children's Hospital, Boston, MA (USA)
- OSTI ID:
- 6207110
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 85:8
- Country of Publication:
- United States
- Language:
- English
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BLOOD COAGULATION FACTORS
BIOLOGICAL EVOLUTION
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AMINO ACID SEQUENCE
GENETIC MAPPING
RECOMBINANT DNA
DNA SEQUENCING
ADHESION
AUTORADIOGRAPHY
CELL DIFFERENTIATION
ELECTROPHORESIS
GUINEA PIGS
LEUKOCYTES
LYMPHOCYTES
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BODY FLUIDS
CARBOHYDRATES
COAGULANTS
CONNECTIVE TISSUE CELLS
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HEMATOLOGIC AGENTS
MAMMALS
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MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RODENTS
SACCHARIDES
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STRUCTURAL CHEMICAL ANALYSIS
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550401* - Genetics- Tracer Techniques