Biotechnology and genetic optimization of fast-growing hardwoods
- NPI, Salt Lake City, UT (United States)
A biotechnology research program was initiated to develop new clones of fast-growing Populus clones resistant to the herbicide glyphosate and resistant to the leaf-spot and canker disease caused by the fungus Septoria musiva. Glyphosate-resistant callus was selected from stem segments cultured in vitro on media supplemented with the herbicide. Plants were regenerated from the glyphosate-resistant callus tissue. A portion of plants reverted to a glyphosate susceptible phenotype during organogenesis. A biologically active filtrate was prepared from S. musiva and influenced fresh weight of Populus callus tissue. Disease-resistant plants were produced through somaclonal variation when shoots developed on stem internodes cultured in vitro. Plantlets were screened for disease symptoms after spraying with a suspension of fungal spores. A frequency of 0.83 percent variant production was observed. Genetically engineered plants were produced after treatment of plant tissue with Agrobacterium tumefasciens strains carrying plasmid genes for antibiotic resistance. Transformers were selected on media enriched with the antibiotic, kanamycin. Presence of foreign DNA was confirmed by Southern blot analysis. Protoplasts of popular were produced but did not regenerate into plant organs. 145 refs., 12 figs., 36 tabs.
- Research Organization:
- New York State Energy Research and Development Authority, New York, NY (United States); Gas Research Inst., Chicago, IL (United States); New York Gas Group, NY (United States); NPI, Salt Lake City, UT (United States)
- Sponsoring Organization:
- NYSERDA; New York State Energy Research and Development Authority, Albany, NY (United States)
- OSTI ID:
- 6199269
- Report Number(s):
- NYSERDA-91-8; ON: TI92002650
- Country of Publication:
- United States
- Language:
- English
Similar Records
Proteomics reveals pathways linked to septoria canker resistance and susceptibility in Populus trichocarpa
Multiplex
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
FUNGAL DISEASES
DISEASE RESISTANCE
HERBICIDES
TOLERANCE
POPLARS
GENETIC ENGINEERING
AGAR
BIOLOGICAL ADAPTATION
BIOTECHNOLOGY
CELL CULTURES
CELL TRANSFORMATIONS
CLONE CELLS
CLONING
CULTIVATION TECHNIQUES
DNA-CLONING
GENETIC VARIABILITY
ONTOGENESIS
PLANT CELLS
PLANT GROWTH
PLANT GROWTH REGULATORS
PLASMIDS
PROGRESS REPORT
BIOLOGICAL VARIABILITY
CARBOHYDRATES
CELL CONSTITUENTS
COLLOIDS
DISEASES
DISPERSIONS
DNA HYBRIDIZATION
DOCUMENT TYPES
GROWTH
HYBRIDIZATION
INFECTIOUS DISEASES
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC COMPOUNDS
PESTICIDES
PLANTS
POLYSACCHARIDES
SACCHARIDES
TREES
553000* - Agriculture & Food Technology
550200 - Biochemistry
550400 - Genetics
090800 - Biomass Fuels- Production- (1990-)