Characterization of noncovalent metal-oligonucleotide complexes
- Pacific Northwest Lab., Richland, WA (United States)
- California Institute of Technology, Pasadena, CA (United States)
Transition metal complexes are being used as probes to determine the conformational structure of oligonucleotides to recognize specific binding sites, and to probe the capability for electron-transfer reactions in DNA strands. For some of the studies transition metal complexes, such as [Ru(phen){sub 2}dppz]{sup 2+}, are tethered to the 5{prime} end of an oligonucleotide chain for use as a spectroscopic probe for the sequence specific detection of oligonucleotides. In other studies, noncovalent interactions, such as intercalation and hydrophobic groove binding, of the metal complexes with oligonucleotide duplexes are used to probe specific DNA sequence and structure. Recent developments with electrospray ionization mass spectrometry (ESI-MS) for the analysis of a wide variety of biomolecules, including oligonucleotides, and importantly the analysis of noncovalent associations has prompted this union of bioinorganic chemistry and mass spectrometry. Presented here are the preliminary results from the use of ESI-MS for the characterization of transition metal-oligonucleotide systems.
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 61959
- Report Number(s):
- CONF-9405234-; TRN: 95:003327-0094
- Resource Relation:
- Conference: 42. American Society of Mass Spectrometry (ASMS) conference on mass spectrometry and allied topics, Chicago, IL (United States), 29 May - 3 Jun 1994; Other Information: PBD: 1994; Related Information: Is Part Of Proceedings of the 42nd ASMS conference on mass spectrometry and allied topics; PB: 1233 p.
- Country of Publication:
- United States
- Language:
- English
Similar Records
Observation of duplex DNA-drug noncovalent complexes by electrospray ionization mass spectrometry
Detection and characterization of small molecule/oligonucleotide duplex noncovalent complexes by electrospray ionization mass spectrometry