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Title: Capillary isotachophoresis mass spectrometry

Abstract

The on-line combination of capillary isotachophoresis (CITP) with mass spectrometry is demonstrated for the first time. The CITP/MS interface is based upon electrospray ionization and is identical with that developed previously for capillary zone electrophoresis (CZE)/MS. Separations were conducted in untreated 100 ..mu..m i.d. fused silica capillaries having lengths of 0.6-2.5 m, at voltages up to 35 kV. The method involves elution of the leading electrolyte to the electrospray source followed by a sequence of separated analyte bands (if sufficient time is provided for development) and, finally, the trailing electrolyte. The CITP/MS was demonstrated to allow very high resolution separations of quaternary phosphonium ions and other ionic substances having very small differences in electrophoretic mobilities. Nearly ideal band shapes are obtained in most separations despite the presence of electroosmotic flow. The potential for application to very dilute sample solutions is demonstrated by detection of analytes having 10/sup /minus/9/ M concentrations, with signal to noise ratios of approximately 10/sup 2/ for some components, which as at least 2 orders of magnitude better than CZE/MS. CITP/MS appears to be an attractive complement to CZE/MS for dilute (low ionic strength) solutions since much greater sample sizes can be addressed without loss of efficiency.

Authors:
; ;
Publication Date:
Research Org.:
Pacific Northwest Lab., Richland, WA (USA)
OSTI Identifier:
6190260
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Journal Article
Journal Name:
Anal. Chem.; (United States)
Additional Journal Information:
Journal Volume: 61:3
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; AMINO ACIDS; ISOTACHOPHORESIS; MASS SPECTROSCOPY; AMMONIUM COMPOUNDS; DOPAMINE; PHOSPHORUS COMPOUNDS; SALTS; CAPILLARIES; CATIONS; EFFICIENCY; EQUIPMENT INTERFACES; EXPERIMENTAL DATA; MIXTURES; QUANTITATIVE CHEMICAL ANALYSIS; RESOLUTION; SEPARATION PROCESSES; SOLUTIONS; TRACE AMOUNTS; AMINES; AROMATICS; AUTONOMIC NERVOUS SYSTEM AGENTS; BLOOD VESSELS; BODY; CARBOXYLIC ACIDS; CARDIOTONICS; CARDIOVASCULAR AGENTS; CARDIOVASCULAR SYSTEM; CHARGED PARTICLES; CHEMICAL ANALYSIS; DATA; DISPERSIONS; DRUGS; ELECTROPHORESIS; HYDROXY COMPOUNDS; INFORMATION; IONS; NEUROREGULATORS; NUMERICAL DATA; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PHENOLS; POLYPHENOLS; SPECTROSCOPY; SYMPATHOMIMETICS; 400105* - Separation Procedures; 400102 - Chemical & Spectral Procedures

Citation Formats

Udseth, H R, Loo, J A, and Smith, R D. Capillary isotachophoresis mass spectrometry. United States: N. p., 1989. Web. doi:10.1021/ac00178a008.
Udseth, H R, Loo, J A, & Smith, R D. Capillary isotachophoresis mass spectrometry. United States. https://doi.org/10.1021/ac00178a008
Udseth, H R, Loo, J A, and Smith, R D. Wed . "Capillary isotachophoresis mass spectrometry". United States. https://doi.org/10.1021/ac00178a008.
@article{osti_6190260,
title = {Capillary isotachophoresis mass spectrometry},
author = {Udseth, H R and Loo, J A and Smith, R D},
abstractNote = {The on-line combination of capillary isotachophoresis (CITP) with mass spectrometry is demonstrated for the first time. The CITP/MS interface is based upon electrospray ionization and is identical with that developed previously for capillary zone electrophoresis (CZE)/MS. Separations were conducted in untreated 100 ..mu..m i.d. fused silica capillaries having lengths of 0.6-2.5 m, at voltages up to 35 kV. The method involves elution of the leading electrolyte to the electrospray source followed by a sequence of separated analyte bands (if sufficient time is provided for development) and, finally, the trailing electrolyte. The CITP/MS was demonstrated to allow very high resolution separations of quaternary phosphonium ions and other ionic substances having very small differences in electrophoretic mobilities. Nearly ideal band shapes are obtained in most separations despite the presence of electroosmotic flow. The potential for application to very dilute sample solutions is demonstrated by detection of analytes having 10/sup /minus/9/ M concentrations, with signal to noise ratios of approximately 10/sup 2/ for some components, which as at least 2 orders of magnitude better than CZE/MS. CITP/MS appears to be an attractive complement to CZE/MS for dilute (low ionic strength) solutions since much greater sample sizes can be addressed without loss of efficiency.},
doi = {10.1021/ac00178a008},
url = {https://www.osti.gov/biblio/6190260}, journal = {Anal. Chem.; (United States)},
number = ,
volume = 61:3,
place = {United States},
year = {1989},
month = {2}
}