Transformation of halogen-, alkyl-, and alkoxy-substituted anilines by a lactase of Trametes versicolor
The lactase of the fungus Trametes versicolor was able to polymerize various halogen-, alkyl-, and alkoxy-substituted anilines, showing substrate specificity similar to that of horseradish peroxidase, whereas the lactase of Rhizoctonia praticola was active only with p-methoxyaniline. The substrate specificities of the enzymes were determined by using gas chromatography to measure the decrease in substrate concentration during incubation. With p-chloroaniline as the substrate, the peroxidase and the Trametes lactase showed maximum activity near pH 4.2. The transformation of this substrate gave rise to a number of oligomers, ranging from dimers to pentamers, as determined by mass spectrometry. The product profiles obtained by high-pressure liquid chromatography were similar for the two enzymes. A chemical reaction was observed between p-chloroaniline and an enzymatically formed dimer, resulting in the formation of a trimer. All three enzymes oxidized p-methoxyaniline to 2-amino-5-p-anisidinobenzoquinone di-p-methoxyphenylimine, but only the T. versicolor lactase and the peroxidase caused the formation of a pentamer (2,5-di-p-anisidinobenzoquinone di-p-methoxyphenylimine). These results demonstrate that in addition to horseradish peroxidase, a T. versicolor lactase can also polymerize aniline derivatives.
- Research Organization:
- Pennsylvania State Univ., University Park
- OSTI ID:
- 6187948
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 49:5
- Country of Publication:
- United States
- Language:
- English
Similar Records
Molecular analysis of the biological bleaching of kraft pulps by Trametes versicolor
Rapid characterization of the activities of lignin-modifying enzymes based on nanostructure-initiator mass spectrometry (NIMS)
Related Subjects
ANILINE
METABOLISM
HYDROLASES
ENZYME ACTIVITY
PEROXIDASES
BIOCHEMICAL REACTION KINETICS
COMPARATIVE EVALUATIONS
FUNGI
GAS CHROMATOGRAPHY
LIQUID COLUMN CHROMATOGRAPHY
SUBSTRATES
AMINES
AROMATICS
CHROMATOGRAPHY
ENZYMES
KINETICS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PLANTS
REACTION KINETICS
SEPARATION PROCESSES
560303* - Chemicals Metabolism & Toxicology- Plants- (-1987)