Comparisons of fixation of heat, radiation, and heat plus radiation damage by anisotonic sodium chloride solutions
Heat treatment at temperatures greater than 40 degrees C synergistically enhanced damage produced by ionizing radiation. Researchers experiments indicated that radiation damage in exponentially growing Chinese hamster cells could be fixed in a dose-dependent manner by postirradiation treatment with both hypertonic and hypotonic NaCl solutions. At a 1,000-rad dose level, survival could be depressed by a factor of about 260. For various treatments at either 42 or 45 degrees C, exposure after heating to anisotonic solutions did not result in the fixation of heat damage. When cells were heated at 45 degrees C for 5 minutes and irradiated with 500 rad before or after heating or given 500 rad without heating and then exposed to 0.05 M NaCl solutions for 120 minutes, survival was reduced by factors of 875, 667, and 12, respectively. For heat treatments at lower temperatures, such as 41.5 or 42 degrees C, less damage fixation for the combined treatments was observed. The data indicated that heat and radiation damage were different and damage from the combined treatments was not the same for low- and high-treatment temperatures.
- Research Organization:
- Medical Biophysics Branch, Atomic Energy of Canada Limited, Pinawa, Manitoba, Canada
- OSTI ID:
- 6187136
- Journal Information:
- NIDA Res. Monogr.; (United States), Vol. 61
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
RADIOSENSITIVITY
HYPERTHERMIA
RADIOSENSITIVITY EFFECTS
BIOLOGICAL EFFECTS
CELL CULTURES
COMPARATIVE EVALUATIONS
DOSE-RESPONSE RELATIONSHIPS
HAMSTERS
QUANTITY RATIO
RESPONSE MODIFYING FACTORS
SODIUM CHLORIDES
SURVIVAL CURVES
TEMPERATURE EFFECTS
USA
X RADIATION
ALKALI METAL COMPOUNDS
ANIMALS
BODY TEMPERATURE
CHLORIDES
CHLORINE COMPOUNDS
ELECTROMAGNETIC RADIATION
HALIDES
HALOGEN COMPOUNDS
IONIZING RADIATIONS
MAMMALS
NORTH AMERICA
RADIATIONS
RODENTS
SODIUM COMPOUNDS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
560201 - Thermal Effects- Cells- (-1987)