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Title: Copper absorption from human milk, cow's milk, and infant formulas using a suckling rat model

Abstract

Since copper deficiency is known to occur during infancy, it becomes important to assess copper uptake from various infant diets. The authors have investigated the uptake of copper from human milk, cow's milk, cow's milk formulas, cereal/milk formula and soy formula, compensating for the decay of /sup 64/Cu and using the suckling rat as a model. Radiocopper was added to the diet in trace amounts. Ultracentrifugation, ultrafiltration, and gel filtration were used to show that the added /sup 64/Cu bound to milk fractions and individual binding compounds in a manner analogous to the distribution of native copper, thus validating the use of extrinsically labeled diets. Labeled diets were intubated into 14-day-old suckling rats. Animals were killed after 6 h and tissues removed and counted. Liver copper uptake was 25% from human milk, 23% from cow's milk formula, 18% from cow's milk, 17% from premature (cow's milk based) infant formula, 17% from cereal/milk formula and 10% from soy formula. These results show that the rat pup model may provide a rapid, inexpensive, and sensitive method to assay bioavailability of copper from infant foods.

Authors:
; ;
Publication Date:
Research Org.:
Univ. of California, Davis
OSTI Identifier:
6185980
Resource Type:
Journal Article
Journal Name:
Am. J. Clin. Nutr.; (United States)
Additional Journal Information:
Journal Volume: 5
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 62 RADIOLOGY AND NUCLEAR MEDICINE; COPPER 64; TISSUE DISTRIBUTION; UPTAKE; COPPER COMPOUNDS; BIOLOGICAL AVAILABILITY; COWS; LIVER; MILK; RATS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BODY; BODY FLUIDS; CATTLE; COPPER ISOTOPES; DIGESTIVE SYSTEM; DISTRIBUTION; DOMESTIC ANIMALS; ELECTRON CAPTURE RADIOISOTOPES; FOOD; GLANDS; HOURS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; ISOTOPES; MAMMALS; MATERIALS; NUCLEI; ODD-ODD NUCLEI; ORGANS; RADIOISOTOPES; RODENTS; RUMINANTS; TRANSITION ELEMENT COMPOUNDS; VERTEBRATES; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987); 550601 - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Loennerdal, B B, Bell, J G, and Keen, C L. Copper absorption from human milk, cow's milk, and infant formulas using a suckling rat model. United States: N. p., 1985. Web.
Loennerdal, B B, Bell, J G, & Keen, C L. Copper absorption from human milk, cow's milk, and infant formulas using a suckling rat model. United States.
Loennerdal, B B, Bell, J G, and Keen, C L. Fri . "Copper absorption from human milk, cow's milk, and infant formulas using a suckling rat model". United States.
@article{osti_6185980,
title = {Copper absorption from human milk, cow's milk, and infant formulas using a suckling rat model},
author = {Loennerdal, B B and Bell, J G and Keen, C L},
abstractNote = {Since copper deficiency is known to occur during infancy, it becomes important to assess copper uptake from various infant diets. The authors have investigated the uptake of copper from human milk, cow's milk, cow's milk formulas, cereal/milk formula and soy formula, compensating for the decay of /sup 64/Cu and using the suckling rat as a model. Radiocopper was added to the diet in trace amounts. Ultracentrifugation, ultrafiltration, and gel filtration were used to show that the added /sup 64/Cu bound to milk fractions and individual binding compounds in a manner analogous to the distribution of native copper, thus validating the use of extrinsically labeled diets. Labeled diets were intubated into 14-day-old suckling rats. Animals were killed after 6 h and tissues removed and counted. Liver copper uptake was 25% from human milk, 23% from cow's milk formula, 18% from cow's milk, 17% from premature (cow's milk based) infant formula, 17% from cereal/milk formula and 10% from soy formula. These results show that the rat pup model may provide a rapid, inexpensive, and sensitive method to assay bioavailability of copper from infant foods.},
doi = {},
url = {https://www.osti.gov/biblio/6185980}, journal = {Am. J. Clin. Nutr.; (United States)},
number = ,
volume = 5,
place = {United States},
year = {1985},
month = {11}
}