sup 1 H and sup 31 P nuclear magnetic resonance investigation of the interaction between 2,3-diphosphoglycerate and human normal adult hemoglobin
- Carnegie Mellon Univ., Pittsburgh, PA (USA)
High-resolution {sup 1}H and {sup 31}P nuclear magnetic resonance spectroscopy has been used to investigate the binding of 2,3-diphosphoglycerate to human normal adult hemoglobin and the molecular interactions involved in the allosteric effect of the 2,3-diphosphoglycerate molecule on hemoglobin. Individual hydrogen ion NMR titration curves have been obtained for 22-26 histidyl residues of hemoglobin and for each phosphate group of 2,3-diphosphoglycerate with hemoglobin in both the deoxy and carbonmonoxy forms. The results indicate that 2,3-diphosphoglycerate binds to deoxyhemoglobin at the central cavity between the two {beta} chains and the binding involves the {beta}2-histidyl residues. Moreover, the results suggest that the binding site of 2,3-diphosphoglycerate to carbonmonoxyhemoglobin contains the same (or at least some of the same) amino acid residues responsible for binding in the deoxy form. As a result of the specific interactions with 2,3-diphosphoglycerate, the {beta}2-histidyl residues make a significant contribution to the alkaline Bohr effect under these experimental conditions. These results give the first experimental demonstration that long-range electrostatic and/or conformation effects of the binding could play an important role in the allosteric effect of 2,3-diphosphoglycerate on hemoglobin. The {sup 31}P nuclear magnetic resonance titration data for each phosphate group of 2,3-diphosphoglycerate have been used to calculate the pK values of the phosphate groups in 2,3-diphosphoglycerate bound to deoxy- and carbon-monoxyhemoglobin and the proton uptake by 2,3-diphosphoglycerate upon ligand binding to hemoglobin.
- OSTI ID:
- 6170335
- Journal Information:
- Biochemistry; (USA), Vol. 29:14; ISSN 0006-2960
- Country of Publication:
- United States
- Language:
- English
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CARBOXYLIC ACID SALTS
NUCLEAR MAGNETIC RESONANCE
HEMOGLOBIN
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ERYTHROCYTES
GLYCERIC ACID
HISTIDINE
LIGANDS
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NMR SPECTRA
OXYGEN
PH VALUE
PHOSPHORUS 31
PROTONS
AGE GROUPS
AMINO ACIDS
ANIMALS
AZOLES
BARYONS
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BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
ELEMENTARY PARTICLES
ELEMENTS
FERMIONS
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HADRONS
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HYDROXY ACIDS
IMIDAZOLES
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LIGHT NUCLEI
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MAMMALS
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NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
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550601* - Medicine- Unsealed Radionuclides in Diagnostics