Recovery of immune functions in dogs after total body irradiation and transplantation of autologous blood or bone marrow cells

Journal Article · Tue Oct 01 00:00:00 EDT 1985 · Exp. Hematol.; (United States)

OSTI ID:6168777

Pruemmer, O R; Raghavachar, A; Fliedner, T M

The restoration of immune functions was followed in dogs for 101 days after fractionated total body irradiation and autologous transfusion of peripheral blood leukocytes (PBL) or bone marrow (BM) cells. Median numbers of 0.9 X 10(5) granulocyte-macrophage progenitor cells per kilogram of body weight were transferred in either group of recipients. The following parameters recovered more rapidly in PBL recipients as opposed to BM recipients: total blood lymphocyte, T- and B-cell counts, serum levels of immunoglobulins IgM and IgA, in vitro blastogenic responses after stimulation with concanavalin A and pokeweed mitogen, and in vitro plasma cell formation after polyclonal B-cell activation with pokeweed mitogen with or without lipopolysaccharide. No major differences were noted for the restoration of serum IgG levels. Circulating lymphocyte and T-cell numbers remained subnormal for more than three months in both groups, whereas B-cell numbers and serum levels of IgA continued to be depressed in BM recipients only. Thus, autologous PBL restored immune functions more rapidly than did BM. Transplantation of PBL, alone or in addition to autologous BM, might also shorten the period of immunodeficiency after cytoreduction in a variety of malignancies in man.

• Research Organization: Univ. of Ulm, Germany, F.R.
• OSTI ID: 6168777
• Journal Information: Exp. Hematol.; (United States), Vol. 9
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BONE MARROW CELLS
BIOLOGICAL RECOVERY
TRANSPLANTS
DOGS
IMMUNE REACTIONS
PLASMA CELLS
BIOLOGICAL RADIATION EFFECTS
CONCANAVALIN
FRACTIONATED IRRADIATION
IMMUNITY
IMMUNOGLOBULINS
LEUKOCYTES
LIPOPOLYSACCHARIDES
MITOGENS
WHOLE-BODY IRRADIATION
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
EXTERNAL IRRADIATION
GLOBULINS
HEMAGGLUTININS
IRRADIATION
LIPIDS
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROTEINS
RADIATION EFFECTS
RECOVERY
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals