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Title: Mn++-stimulated /sup 3/H-inositol incorporation in cultured vascular smooth muscle cells: deficiency in cells from spontaneously hypertensive rats

Abstract

Incubation of cultured dissociated aortic smooth muscle cells from Wistar-Kyoto rats (WKY) with Mn++ resulted in a 10-fold stimulation of /sup 3/H-myo-inositol incorporation into membrane phospholipids. The stimulation was temperature and energy dependent. The Mn++ EC50 was 1 mM and maximum stimulation occurred at 10 mM Mn++. Other cations were ineffective. In cells from spontaneously hypertensive rats (SHR), the Mn++ EC50 was unchanged whereas the maximum stimulation of /sup 3/H-inositol incorporation was reduced by 50% compared to cells from parallel WKY cultures. These results suggest that in SHR cultured vascular smooth muscle cells there is a deficiency in an enzymatic process mediating exchange of free inositol with the headgroup inositol of membrane phosphatidylinositol.

Authors:
; ;
Publication Date:
Research Org.:
NHLBI, NIH, Bethesda, MD
OSTI Identifier:
6168431
Resource Type:
Journal Article
Journal Name:
Clin. Exp. Hypertens., Part A; (United States)
Additional Journal Information:
Journal Volume: 7
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; INOSITOL; METABOLISM; MANGANESE; BIOLOGICAL EFFECTS; CATIONS; CELL CULTURES; CELL MEMBRANES; HYPERTENSION; MUSCLES; PHOSPHOLIPIDS; RATS; TEMPERATURE DEPENDENCE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMALS; CARBOHYDRATES; CARDIOVASCULAR DISEASES; CELL CONSTITUENTS; CHARGED PARTICLES; DISEASES; ELEMENTS; ESTERS; INOSITOLS; IONS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LIPIDS; MAMMALS; MEMBRANES; METALS; MONOSACCHARIDES; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; RODENTS; SACCHARIDES; SYMPTOMS; TRANSITION ELEMENTS; VASCULAR DISEASES; VERTEBRATES; 560301* - Chemicals Metabolism & Toxicology- Cells- (-1987); 550901 - Pathology- Tracer Techniques

Citation Formats

Buck, S H, Nabika, T, and Lovenberg, W. Mn++-stimulated /sup 3/H-inositol incorporation in cultured vascular smooth muscle cells: deficiency in cells from spontaneously hypertensive rats. United States: N. p., 1985. Web.
Buck, S H, Nabika, T, & Lovenberg, W. Mn++-stimulated /sup 3/H-inositol incorporation in cultured vascular smooth muscle cells: deficiency in cells from spontaneously hypertensive rats. United States.
Buck, S H, Nabika, T, and Lovenberg, W. 1985. "Mn++-stimulated /sup 3/H-inositol incorporation in cultured vascular smooth muscle cells: deficiency in cells from spontaneously hypertensive rats". United States.
@article{osti_6168431,
title = {Mn++-stimulated /sup 3/H-inositol incorporation in cultured vascular smooth muscle cells: deficiency in cells from spontaneously hypertensive rats},
author = {Buck, S H and Nabika, T and Lovenberg, W},
abstractNote = {Incubation of cultured dissociated aortic smooth muscle cells from Wistar-Kyoto rats (WKY) with Mn++ resulted in a 10-fold stimulation of /sup 3/H-myo-inositol incorporation into membrane phospholipids. The stimulation was temperature and energy dependent. The Mn++ EC50 was 1 mM and maximum stimulation occurred at 10 mM Mn++. Other cations were ineffective. In cells from spontaneously hypertensive rats (SHR), the Mn++ EC50 was unchanged whereas the maximum stimulation of /sup 3/H-inositol incorporation was reduced by 50% compared to cells from parallel WKY cultures. These results suggest that in SHR cultured vascular smooth muscle cells there is a deficiency in an enzymatic process mediating exchange of free inositol with the headgroup inositol of membrane phosphatidylinositol.},
doi = {},
url = {https://www.osti.gov/biblio/6168431}, journal = {Clin. Exp. Hypertens., Part A; (United States)},
number = ,
volume = 7,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 1985},
month = {Tue Jan 01 00:00:00 EST 1985}
}