skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Effects of carbon dioxide and pH variations in vitro on blood respiratory functions, red blood cell volume, transmembrane pH gradients, and sickling in sickle cell anemia

Abstract

An examination was made of the O/sub 2/ affinity, Bohr effect, transmembrane pH gradient, mean cell hemoglobin concentration, and red blood cell sickling at half O/sub 2/ saturation in whole sickle cell (SS) and normal (AA) blood during CO/sub 2/ titration and acid-base titration at three Pco/sub 2/ levels, 10, 40, and 80 mm Hg. The CO/sub 2/-induced Bohr effect of SS blood was considerably larger than normal (maximum, 0.91, referred to cell pH) and similar to that found with acid-base titration at Pco/sub 2/ of 40. In contrast to AA blood, SS blood showed an increased O/sub 2/ affinity when Pco/sub 2/ was raised from 40 to 80, and at half O/sub 2/ saturation showed biphasic or sigmoid Bohr curves, a fall in transmembrane pH gradient with rising Pco/sub 2/, and an absence of the normal cell volume increase at low pH and Pco/sub 2/. Sickling of SS cells at half O/sub 2/ saturation was partly inhibited by increasing Pc/sub 2/, particularly in the higher pH ranges. These complex differences in the behavior of SS blood are interpreted in terms of the balancing of several effects: the lowering of hemoglobin O/sub 2/-affinity by polymerization, low pH and increased CO/submore » 2/ binding, inhibition of hemoglobin S polymerization by CO/sub 2/ binding to ..beta../sup s/-chain amino termini, differences between hemoglobin S and A in competitive binding of CO/sub 2/ and 2,3-diphosphoglycerate at different pH levels, and an increased net negative charge exhibited by intracellular deoxyhemoglobin S polymers. From a clinical standpoint, in the absence of hypoxia or acidosis, an increased blood Pco/sub 2/ might have a beneficial effect by inhibiting red blood cell sickling, whereas a metabolic acidosis, with low blood pH and Pco/sub 2/, would be very hazardous.« less

Authors:
;
Publication Date:
Research Org.:
Albert Einstein Coll. of Medicine, Bronx, NY
OSTI Identifier:
6166141
Resource Type:
Journal Article
Journal Name:
J. Lab. Clin. Med.; (United States)
Additional Journal Information:
Journal Volume: 104:2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CARBON DIOXIDE; BIOLOGICAL EFFECTS; ERYTHROCYTES; MORPHOLOGY; PH VALUE; VARIATIONS; SICKLE CELL ANEMIA; BLOOD; IN VITRO; ANEMIAS; BIOLOGICAL MATERIALS; BLOOD CELLS; BODY FLUIDS; CARBON COMPOUNDS; CARBON OXIDES; CHALCOGENIDES; DISEASES; HEMIC DISEASES; MATERIALS; OXIDES; OXYGEN COMPOUNDS; SYMPTOMS; 560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)

Citation Formats

Ueda, Y, and Bookchin, R M. Effects of carbon dioxide and pH variations in vitro on blood respiratory functions, red blood cell volume, transmembrane pH gradients, and sickling in sickle cell anemia. United States: N. p., 1984. Web.
Ueda, Y, & Bookchin, R M. Effects of carbon dioxide and pH variations in vitro on blood respiratory functions, red blood cell volume, transmembrane pH gradients, and sickling in sickle cell anemia. United States.
Ueda, Y, and Bookchin, R M. 1984. "Effects of carbon dioxide and pH variations in vitro on blood respiratory functions, red blood cell volume, transmembrane pH gradients, and sickling in sickle cell anemia". United States.
@article{osti_6166141,
title = {Effects of carbon dioxide and pH variations in vitro on blood respiratory functions, red blood cell volume, transmembrane pH gradients, and sickling in sickle cell anemia},
author = {Ueda, Y and Bookchin, R M},
abstractNote = {An examination was made of the O/sub 2/ affinity, Bohr effect, transmembrane pH gradient, mean cell hemoglobin concentration, and red blood cell sickling at half O/sub 2/ saturation in whole sickle cell (SS) and normal (AA) blood during CO/sub 2/ titration and acid-base titration at three Pco/sub 2/ levels, 10, 40, and 80 mm Hg. The CO/sub 2/-induced Bohr effect of SS blood was considerably larger than normal (maximum, 0.91, referred to cell pH) and similar to that found with acid-base titration at Pco/sub 2/ of 40. In contrast to AA blood, SS blood showed an increased O/sub 2/ affinity when Pco/sub 2/ was raised from 40 to 80, and at half O/sub 2/ saturation showed biphasic or sigmoid Bohr curves, a fall in transmembrane pH gradient with rising Pco/sub 2/, and an absence of the normal cell volume increase at low pH and Pco/sub 2/. Sickling of SS cells at half O/sub 2/ saturation was partly inhibited by increasing Pc/sub 2/, particularly in the higher pH ranges. These complex differences in the behavior of SS blood are interpreted in terms of the balancing of several effects: the lowering of hemoglobin O/sub 2/-affinity by polymerization, low pH and increased CO/sub 2/ binding, inhibition of hemoglobin S polymerization by CO/sub 2/ binding to ..beta../sup s/-chain amino termini, differences between hemoglobin S and A in competitive binding of CO/sub 2/ and 2,3-diphosphoglycerate at different pH levels, and an increased net negative charge exhibited by intracellular deoxyhemoglobin S polymers. From a clinical standpoint, in the absence of hypoxia or acidosis, an increased blood Pco/sub 2/ might have a beneficial effect by inhibiting red blood cell sickling, whereas a metabolic acidosis, with low blood pH and Pco/sub 2/, would be very hazardous.},
doi = {},
url = {https://www.osti.gov/biblio/6166141}, journal = {J. Lab. Clin. Med.; (United States)},
number = ,
volume = 104:2,
place = {United States},
year = {Wed Aug 01 00:00:00 EDT 1984},
month = {Wed Aug 01 00:00:00 EDT 1984}
}