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Title: Tritiated uracil, tritiated thymidine, and bromodeoxyuridine induced mutations in eucaryotic cells. [Saccharomyces cerevisiae, gamma radiation]

Abstract

The induction of gene conversion at the ARG-4 locus in strain BZ34 of Saccharomyces cerevisiae was examined after the cells incorporated y-/sup 3/H uracil under optimum growth conditions for 16 hours, and then received damage at 4/sup 0/C from tritium decays at very low dose rates of 1.4 to 27.6 tritium decays per hour. The results were compared to the results of gene conversion induced by /sup 60/Co. The induction of resistance to 6TG in Chinese hamster ovary (CHO) cells has been studied after incorporation of /sup 3/H-methyl thymidine, 6-/sup 3/H-thymidine, and bromodeoxyuridine under several experimental conditions. The induction of mutations by incorporated 6-/sup 3/H-thymidine is about three times as effective as the induction of mutations by tritiated-methyl thymidine. These results suggest that the determination of the RBE for tritium decays in model eucaryotic systems like yeast and cultured Chinese hamster cells will be influenced by the precise experimental conditions employed. In particular, experiments with mammalian cells will be affected by hot times for mutagenesis in the cell cycle and hot positions within the DNA in the nucleus, and also by the position of tritium decay within the DNA-incorporated molecule.

Authors:
; ;
Publication Date:
Research Org.:
California Univ., Berkeley (USA). Lawrence Berkeley Lab.; California Univ., San Francisco (USA). Lab. of Radiobiology; Institut du Radium, 91 - Orsay (France)
OSTI Identifier:
6164908
Report Number(s):
LBL-8811; CONF-790325-4; IAEA-SM-237/39
TRN: 79-010941
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Conference
Resource Relation:
Conference: International symposium on biological implications of radionuclides released from nuclear industries, Vienna, Austria, 26 Mar 1979
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ANIMAL CELLS; BIOLOGICAL RADIATION EFFECTS; MUTATIONS; BUDR; GENETIC EFFECTS; RADIOINDUCTION; SACCHAROMYCES CEREVISIAE; THYMIDINE; TRITIUM; RBE; URACILS; EXPERIMENTAL DATA; GAMMA RADIATION; GENES; GRAPHS; ANTIMETABOLITES; ANTIMITOTIC DRUGS; AZINES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BROMOURACILS; DATA; DATA FORMS; DRUGS; ELECTROMAGNETIC RADIATION; FUNGI; HETEROCYCLIC COMPOUNDS; HYDROGEN ISOTOPES; HYDROXY COMPOUNDS; INFORMATION; IONIZING RADIATIONS; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; NUCLEI; NUCLEOSIDES; NUCLEOTIDES; NUMERICAL DATA; ODD-EVEN NUCLEI; ORGANIC BROMINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PLANTS; PYRIMIDINES; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; RIBOSIDES; SACCHAROMYCES; YEARS LIVING RADIOISOTOPES; YEASTS; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987); 560121 - Radiation Effects on Cells- External Source- (-1987)

Citation Formats

Burki, H.J., Moustacchi, E., and Cleaver, J.E. Tritiated uracil, tritiated thymidine, and bromodeoxyuridine induced mutations in eucaryotic cells. [Saccharomyces cerevisiae, gamma radiation]. United States: N. p., 1979. Web.
Burki, H.J., Moustacchi, E., & Cleaver, J.E. Tritiated uracil, tritiated thymidine, and bromodeoxyuridine induced mutations in eucaryotic cells. [Saccharomyces cerevisiae, gamma radiation]. United States.
Burki, H.J., Moustacchi, E., and Cleaver, J.E. Thu . "Tritiated uracil, tritiated thymidine, and bromodeoxyuridine induced mutations in eucaryotic cells. [Saccharomyces cerevisiae, gamma radiation]". United States. https://www.osti.gov/servlets/purl/6164908.
@article{osti_6164908,
title = {Tritiated uracil, tritiated thymidine, and bromodeoxyuridine induced mutations in eucaryotic cells. [Saccharomyces cerevisiae, gamma radiation]},
author = {Burki, H.J. and Moustacchi, E. and Cleaver, J.E.},
abstractNote = {The induction of gene conversion at the ARG-4 locus in strain BZ34 of Saccharomyces cerevisiae was examined after the cells incorporated y-/sup 3/H uracil under optimum growth conditions for 16 hours, and then received damage at 4/sup 0/C from tritium decays at very low dose rates of 1.4 to 27.6 tritium decays per hour. The results were compared to the results of gene conversion induced by /sup 60/Co. The induction of resistance to 6TG in Chinese hamster ovary (CHO) cells has been studied after incorporation of /sup 3/H-methyl thymidine, 6-/sup 3/H-thymidine, and bromodeoxyuridine under several experimental conditions. The induction of mutations by incorporated 6-/sup 3/H-thymidine is about three times as effective as the induction of mutations by tritiated-methyl thymidine. These results suggest that the determination of the RBE for tritium decays in model eucaryotic systems like yeast and cultured Chinese hamster cells will be influenced by the precise experimental conditions employed. In particular, experiments with mammalian cells will be affected by hot times for mutagenesis in the cell cycle and hot positions within the DNA in the nucleus, and also by the position of tritium decay within the DNA-incorporated molecule.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1979},
month = {2}
}

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