skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Developmental co-expression of small molecular weight apolipoprotein B synthesis and triacylglycerol secretion

Abstract

The development of the liver's ability to coordinately express the synthesis and secretion of the two major components of very low density lipoproteins (VLDL): triacylglycerol (TG) and apolipoprotein B (apo B) was examined in cultured hepatocytes obtained from fetal, suckling and adult rats. Hepatocytes from fetal and suckling rats synthesized and secreted TG at rates lower than that displayed by adult cells. When TG synthesis was equalized by adding oleic acid to the culture medium, fetal cells still secreted only 39% as much TG as did adult cells. To determine the basis for the apparent defect in VLDL assembly/secretion displayed by fetal cells, the synthesis and secretion of (TVS)methionine-labeled apo B was quantified by immunoprecipitation. Although adult and fetal cells synthesized and secreted large molecular weight apo B at similar rates, the synthesis and secretion of small molecular weight apo B was 2-fold greater in adult cells. These data suggest that the ability to assemble/secrete VLDL triacylglycerol varies in parallel with the developmental expression of small molecular weight apo B. Furthermore, these studies show the usefulness of the cultured rat hepatocyte model for examining the ontogeny and regulation of VLDL assembly/secretion.

Authors:
; ; ;
Publication Date:
Research Org.:
Duke Univ. Medical Center, Durham, NC
OSTI Identifier:
6156595
Report Number(s):
CONF-870644-
Journal ID: CODEN: FEPRA; TRN: 87-028686
Resource Type:
Conference
Resource Relation:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States); Journal Volume: 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; APOLIPOPROTEINS; BIOSYNTHESIS; TRIGLYCERIDES; SECRETION; AGE DEPENDENCE; BIOLOGICAL MODELS; LIVER CELLS; METHIONINE; MOLECULAR WEIGHT; RATS; SULFUR 35; TRACER TECHNIQUES; AMINO ACIDS; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; DAYS LIVING RADIOISOTOPES; DRUGS; ESTERS; EVEN-ODD NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; LIPIDS; LIPOPROTEINS; LIPOTROPIC FACTORS; MAMMALS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PROTEINS; RADIOISOTOPES; RODENTS; SOMATIC CELLS; SULFUR ISOTOPES; SYNTHESIS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Coleman, R.A., Haynes, E.B., Sand, T.M., and Davis, R.A. Developmental co-expression of small molecular weight apolipoprotein B synthesis and triacylglycerol secretion. United States: N. p., 1987. Web.
Coleman, R.A., Haynes, E.B., Sand, T.M., & Davis, R.A. Developmental co-expression of small molecular weight apolipoprotein B synthesis and triacylglycerol secretion. United States.
Coleman, R.A., Haynes, E.B., Sand, T.M., and Davis, R.A. Fri . "Developmental co-expression of small molecular weight apolipoprotein B synthesis and triacylglycerol secretion". United States.
@article{osti_6156595,
title = {Developmental co-expression of small molecular weight apolipoprotein B synthesis and triacylglycerol secretion},
author = {Coleman, R.A. and Haynes, E.B. and Sand, T.M. and Davis, R.A.},
abstractNote = {The development of the liver's ability to coordinately express the synthesis and secretion of the two major components of very low density lipoproteins (VLDL): triacylglycerol (TG) and apolipoprotein B (apo B) was examined in cultured hepatocytes obtained from fetal, suckling and adult rats. Hepatocytes from fetal and suckling rats synthesized and secreted TG at rates lower than that displayed by adult cells. When TG synthesis was equalized by adding oleic acid to the culture medium, fetal cells still secreted only 39% as much TG as did adult cells. To determine the basis for the apparent defect in VLDL assembly/secretion displayed by fetal cells, the synthesis and secretion of (TVS)methionine-labeled apo B was quantified by immunoprecipitation. Although adult and fetal cells synthesized and secreted large molecular weight apo B at similar rates, the synthesis and secretion of small molecular weight apo B was 2-fold greater in adult cells. These data suggest that the ability to assemble/secrete VLDL triacylglycerol varies in parallel with the developmental expression of small molecular weight apo B. Furthermore, these studies show the usefulness of the cultured rat hepatocyte model for examining the ontogeny and regulation of VLDL assembly/secretion.},
doi = {},
journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 46:6,
place = {United States},
year = {Fri May 01 00:00:00 EDT 1987},
month = {Fri May 01 00:00:00 EDT 1987}
}

Conference:
Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this conference proceeding.

Save / Share: