Identification and characterization of an angiotensin II receptor on cultured bovine adrenal chromaffin cells
The presence of an angiotensin II receptor on cultured bovine adrenal chromaffin cells was demonstrated by radioligand binding. A single class of finding sites with a K/sub D/ of 0.7 nM was characterized. The use of radioligands also allows the localization of receptors by autoradiography. Autoradiography demonstrated that approximately 50% of the isolated cells bound angiotensin II. It was of interest to see if angiotensin II bound to a cell that possessed a certain phenotype. In order to evaluate this possibility a technique was developed that combined autoradiography and immunocytochemistry. Results indicated that angiotensin II binding sites were not localized preferentially to either norepinephrine or epinephrine cells. Binding of angiotensin II was associated with the release of intracellular catecholamine stores. Cells were pre-loaded with /sup 3/H-norepinephrine and secretion was monitored by following radioactivity released into the supernatant. Alternatively, release of endogenous catecholamines was determined by fluorometric assay.
- Research Organization:
- California Univ., San Diego (USA)
- OSTI ID:
- 6154723
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANGIOTENSIN
RECEPTORS
CHEMICAL COMPOSITION
ADRENAL GLANDS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
CATECHOLAMINES
CATTLE
LIGANDS
NORADRENALINE
PHENOTYPE
TRITIUM COMPOUNDS
ADRENAL HORMONES
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
DOMESTIC ANIMALS
DRUGS
ENDOCRINE GLANDS
GLANDS
GLOBULINS
HYDROXY COMPOUNDS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
PROTEINS
REACTION KINETICS
RUMINANTS
SYMPATHOMIMETICS
VASOCONSTRICTORS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques