skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Progestin receptor cells in the 8-day-old male and female mouse cerebral cortex: Autoradiographic evidence for a sexual dimorphism in target cell number

Abstract

The present study examined the number and distribution of progestin receptor cells in the 8-day-old male and female cortex and compared cortical labeling with that in the preoptic area and central hypothalamus. Eight-day postnatal mice (four males and four females), treated with estradiol, were each sc injected with 0.32 micrograms/100 g BW (125I)progestin. Brains were frozen 2 h after injection of (125I)progestin, sectioned, and processed for thaw-mount autoradiography. Cells with a nuclear concentration of radioactivity were localized in lamina VI of the lateral cortical regions of the male and female brain, while only a few cortical cells were seen in laminae II, III, and V of the suprarhinal, lateral, and cingulate/paracingulate regions. Comparison of the number of labeled cells revealed that the female cortex contained significantly more labeled cells than the male at three of the four levels investigated. Similarly, the number of target cells was higher in the female medial preoptic nucleus, but not in the arcuate nucleus and ventromedial hypothalamic nucleus, while the distributions of labeled cells in the male and female preoptic/hypothalamic regions were comparable. Injection of unlabeled progesterone or R5020 1 h before (125I)progestin reduced the nuclear concentration of radioactivity in all target regions and verifiedmore » the specificity of (125I)progestin for the progestin receptor. The results of these studies indicate that mouse 8-day-old cortex and preoptic area in the female animal have more progestin receptor cells than those in the male and demonstrate that progestin receptor cells are localized in a region of the cortex known to contain few estrogen target cells. These results further suggest that a sexual dimorphism in progestin cell number may result in a differential effect of progestin on the cortex and preoptic area of the mouse.« less

Authors:
; ; ; ;  [1]
  1. Univ. of North Carolina, Chapel Hill (USA)
Publication Date:
OSTI Identifier:
6152687
Resource Type:
Journal Article
Journal Name:
Endocrinology; (USA)
Additional Journal Information:
Journal Volume: 128:1; Journal ID: ISSN 0013-7227
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BRAIN; AUTORADIOGRAPHY; PROGESTERONE; RECEPTORS; TISSUE DISTRIBUTION; BIOCHEMICAL REACTION KINETICS; ESTRADIOL; IODINE 125; MICE; SEX DEPENDENCE; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; CENTRAL NERVOUS SYSTEM; DAYS LIVING RADIOISOTOPES; DISTRIBUTION; ELECTRON CAPTURE RADIOISOTOPES; ESTRANES; ESTROGENS; HORMONES; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; KETONES; KINETICS; MAMMALS; MEMBRANE PROTEINS; NERVOUS SYSTEM; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PREGNANES; PROTEINS; RADIOISOTOPES; REACTION KINETICS; RODENTS; STEROID HORMONES; STEROIDS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Shughrue, P J, Stumpf, W E, Elger, W, Schulze, P E, and Sar, M. Progestin receptor cells in the 8-day-old male and female mouse cerebral cortex: Autoradiographic evidence for a sexual dimorphism in target cell number. United States: N. p., 1991. Web. doi:10.1210/endo-128-1-87.
Shughrue, P J, Stumpf, W E, Elger, W, Schulze, P E, & Sar, M. Progestin receptor cells in the 8-day-old male and female mouse cerebral cortex: Autoradiographic evidence for a sexual dimorphism in target cell number. United States. https://doi.org/10.1210/endo-128-1-87
Shughrue, P J, Stumpf, W E, Elger, W, Schulze, P E, and Sar, M. Tue . "Progestin receptor cells in the 8-day-old male and female mouse cerebral cortex: Autoradiographic evidence for a sexual dimorphism in target cell number". United States. https://doi.org/10.1210/endo-128-1-87.
@article{osti_6152687,
title = {Progestin receptor cells in the 8-day-old male and female mouse cerebral cortex: Autoradiographic evidence for a sexual dimorphism in target cell number},
author = {Shughrue, P J and Stumpf, W E and Elger, W and Schulze, P E and Sar, M},
abstractNote = {The present study examined the number and distribution of progestin receptor cells in the 8-day-old male and female cortex and compared cortical labeling with that in the preoptic area and central hypothalamus. Eight-day postnatal mice (four males and four females), treated with estradiol, were each sc injected with 0.32 micrograms/100 g BW (125I)progestin. Brains were frozen 2 h after injection of (125I)progestin, sectioned, and processed for thaw-mount autoradiography. Cells with a nuclear concentration of radioactivity were localized in lamina VI of the lateral cortical regions of the male and female brain, while only a few cortical cells were seen in laminae II, III, and V of the suprarhinal, lateral, and cingulate/paracingulate regions. Comparison of the number of labeled cells revealed that the female cortex contained significantly more labeled cells than the male at three of the four levels investigated. Similarly, the number of target cells was higher in the female medial preoptic nucleus, but not in the arcuate nucleus and ventromedial hypothalamic nucleus, while the distributions of labeled cells in the male and female preoptic/hypothalamic regions were comparable. Injection of unlabeled progesterone or R5020 1 h before (125I)progestin reduced the nuclear concentration of radioactivity in all target regions and verified the specificity of (125I)progestin for the progestin receptor. The results of these studies indicate that mouse 8-day-old cortex and preoptic area in the female animal have more progestin receptor cells than those in the male and demonstrate that progestin receptor cells are localized in a region of the cortex known to contain few estrogen target cells. These results further suggest that a sexual dimorphism in progestin cell number may result in a differential effect of progestin on the cortex and preoptic area of the mouse.},
doi = {10.1210/endo-128-1-87},
url = {https://www.osti.gov/biblio/6152687}, journal = {Endocrinology; (USA)},
issn = {0013-7227},
number = ,
volume = 128:1,
place = {United States},
year = {1991},
month = {1}
}