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Title: Determination of genes and phenotypes of bacteria necessary for epiphytic colonization and survival on plants

Abstract

Bacteria that live as epiphytes on the surface of healthy plants are important as plant pathogens, in causing plant frost injury by catalyzing ice formation, and in other processes. The objectives of this study are to determine the traits of these epiphytic bacteria which allow them to grow and/or survive in the hostile leaf surface environment. The genes and phenotypes of a strain of Pseudomonas syringae that are necessary for epiphytic fitness on bean leaves are being determined by an evaluation of the fitness and phenotypes of 5300 individual Tn5-induced insertion mutants. Attention is being placed on several mutants which exhibit no known phenotypic alteration, but which while growing normally on moist leaf surfaces succumb to the stresses associated with dry leaf surfaces, unlike the parental strain. Two genes from stress-sensitive epiphytic fitness mutants that have individually large effects on fitness have been identified in a cosmid library of the parental P. syringae strain by sequence homology to Tn5-containing fragments cloned from mutant strains. A 4.0 kb region subcloned from one cosmid restores the ability of mutant strains to survive drying stress on leaves. Fusions of these regions with a promoterless InaZ gene have been made to determine the regulationmore » of transcription of these regions in situ by measuring the ice nuclei produced in merodiploid strains containing these fusion genes. An Ice{sup {minus}} derivative of the parental P. syringae strain has been made by marker-exchange mutagenesis to allow measurements of ice nuclei in situ in these merodiploids exposed to different conditions on leaves. 11 figs., 1 tab.« less

Authors:
Publication Date:
Research Org.:
California Univ., Berkeley, CA (USA). Dept. of Plant Pathology
Sponsoring Org.:
DOE/ER
OSTI Identifier:
6150634
Report Number(s):
DOE/ER/13518-5
ON: DE91008659
DOE Contract Number:  
FG03-86ER13518
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; ICE; NUCLEATION; MUTANTS; COLONY FORMATION; PSEUDOMONAS; GENETIC ENGINEERING; TRANSPOSONS; MUTAGENESIS; BIOLOGICAL STRESS; BIOTECHNOLOGY; DNA HYBRIDIZATION; DNA-CLONING; GENE REGULATION; GENETIC MAPPING; GENOTYPE; LABELLING; LEAVES; MOLECULAR STRUCTURE; PHASEOLUS; PHENOTYPE; PHOSPHORUS 32; PROGRESS REPORT; BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CLONING; DAYS LIVING RADIOISOTOPES; DOCUMENT TYPES; HYBRIDIZATION; ISOTOPES; LEGUMINOSAE; LIGHT NUCLEI; MAGNOLIOPHYTA; MAGNOLIOPSIDA; MAPPING; MICROORGANISMS; NUCLEI; ODD-ODD NUCLEI; PHOSPHORUS ISOTOPES; PLANTS; RADIOISOTOPES; 550400* - Genetics; 550700 - Microbiology; 553003 - Agriculture & Food Technology- Pest & Disease Control- (1987-)

Citation Formats

Lindow, S E. Determination of genes and phenotypes of bacteria necessary for epiphytic colonization and survival on plants. United States: N. p., 1990. Web.
Lindow, S E. Determination of genes and phenotypes of bacteria necessary for epiphytic colonization and survival on plants. United States.
Lindow, S E. Mon . "Determination of genes and phenotypes of bacteria necessary for epiphytic colonization and survival on plants". United States.
@article{osti_6150634,
title = {Determination of genes and phenotypes of bacteria necessary for epiphytic colonization and survival on plants},
author = {Lindow, S E},
abstractNote = {Bacteria that live as epiphytes on the surface of healthy plants are important as plant pathogens, in causing plant frost injury by catalyzing ice formation, and in other processes. The objectives of this study are to determine the traits of these epiphytic bacteria which allow them to grow and/or survive in the hostile leaf surface environment. The genes and phenotypes of a strain of Pseudomonas syringae that are necessary for epiphytic fitness on bean leaves are being determined by an evaluation of the fitness and phenotypes of 5300 individual Tn5-induced insertion mutants. Attention is being placed on several mutants which exhibit no known phenotypic alteration, but which while growing normally on moist leaf surfaces succumb to the stresses associated with dry leaf surfaces, unlike the parental strain. Two genes from stress-sensitive epiphytic fitness mutants that have individually large effects on fitness have been identified in a cosmid library of the parental P. syringae strain by sequence homology to Tn5-containing fragments cloned from mutant strains. A 4.0 kb region subcloned from one cosmid restores the ability of mutant strains to survive drying stress on leaves. Fusions of these regions with a promoterless InaZ gene have been made to determine the regulation of transcription of these regions in situ by measuring the ice nuclei produced in merodiploid strains containing these fusion genes. An Ice{sup {minus}} derivative of the parental P. syringae strain has been made by marker-exchange mutagenesis to allow measurements of ice nuclei in situ in these merodiploids exposed to different conditions on leaves. 11 figs., 1 tab.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1990},
month = {1}
}

Technical Report:
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