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Title: Formation and action of lignin-modifying enzymes in cultures of Phlebia radiata supplemented with veratric acid

Abstract

Transformation of veratric (3,4-dimethoxybenzoic) acid by the white rot fungus Phlebia radiata was studied to elucidate the role of ligninolytic, reductive, and demeth(ox)ylating enzymes. Under both air and a 100% O{sub 2} atmosphere, with nitrogen limitation and glucose as a carbon source, reducing activity resulted in the accumulation of veratryl alcohol in the medium. When the fungus was cultivated under air, veratric acid caused a rapid increase in laccase (benzenediol:oxygen oxidoreductase; EC 1.10.3.2) production, which indicated that veratic acid was first demethylated, thus providing phenolic compounds for laccase. After a rapid decline in laccase activity, elevated lignin peroxidase (ligninase) activity and manganese-dependent peroxidase production were detected simultaneously with extracellular release of methanol. This indicated apparent demethoxylation. When the fungus was cultivated under a continuous 100% O{sub 2} flow and in the presence of veratric acid, laccase production was markedly repressed, whereas production of lignin peroxidase and degradation of veratryl compounds were clearly enhanced. In all cultures, the increases in lignin peroxidase titers were directly related to veratryl alcohol accumulation. Evolution of {sup 14}CO{sub 2} from 3-O{sup 14}CH{sub 3}-and 4-O{sup 14}CH{sub 3}-labeled veratric acids showed that the position of the methoxyl substituent in the aromatic ring only slightly affected demeth(ox)ylation activity.more » In both cases, more than 60% of the total {sup 14}C was converted to {sup 14}CO{sub 2} under air in 4 weeks, and oxygen flux increased the degradation rate of the {sup 14}C-labeled veratric acids just as it did with unlabeled cultures.« less

Authors:
;  [1]; ;  [2]
  1. Univ. of Helsinki (Finland)
  2. Univ. of Maria Curie-Sklodowska, Lublin (Poland)
Publication Date:
OSTI Identifier:
6143172
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology; (USA)
Additional Journal Information:
Journal Volume: 56:9; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; FUNGI; ENZYME ACTIVITY; LIGNIN; BIODEGRADATION; OXIDOREDUCTASES; BIOCHEMICAL REACTION KINETICS; CARBON 14 COMPOUNDS; TRACER TECHNIQUES; CARBOHYDRATES; CHEMICAL REACTIONS; DECOMPOSITION; ENZYMES; ISOTOPE APPLICATIONS; KINETICS; LABELLED COMPOUNDS; ORGANIC COMPOUNDS; PLANTS; POLYSACCHARIDES; REACTION KINETICS; SACCHARIDES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Lundell, T, Hatakka, A, Leonowicz, A, and Rogalski, J. Formation and action of lignin-modifying enzymes in cultures of Phlebia radiata supplemented with veratric acid. United States: N. p., 1990. Web.
Lundell, T, Hatakka, A, Leonowicz, A, & Rogalski, J. Formation and action of lignin-modifying enzymes in cultures of Phlebia radiata supplemented with veratric acid. United States.
Lundell, T, Hatakka, A, Leonowicz, A, and Rogalski, J. Sat . "Formation and action of lignin-modifying enzymes in cultures of Phlebia radiata supplemented with veratric acid". United States.
@article{osti_6143172,
title = {Formation and action of lignin-modifying enzymes in cultures of Phlebia radiata supplemented with veratric acid},
author = {Lundell, T and Hatakka, A and Leonowicz, A and Rogalski, J},
abstractNote = {Transformation of veratric (3,4-dimethoxybenzoic) acid by the white rot fungus Phlebia radiata was studied to elucidate the role of ligninolytic, reductive, and demeth(ox)ylating enzymes. Under both air and a 100% O{sub 2} atmosphere, with nitrogen limitation and glucose as a carbon source, reducing activity resulted in the accumulation of veratryl alcohol in the medium. When the fungus was cultivated under air, veratric acid caused a rapid increase in laccase (benzenediol:oxygen oxidoreductase; EC 1.10.3.2) production, which indicated that veratic acid was first demethylated, thus providing phenolic compounds for laccase. After a rapid decline in laccase activity, elevated lignin peroxidase (ligninase) activity and manganese-dependent peroxidase production were detected simultaneously with extracellular release of methanol. This indicated apparent demethoxylation. When the fungus was cultivated under a continuous 100% O{sub 2} flow and in the presence of veratric acid, laccase production was markedly repressed, whereas production of lignin peroxidase and degradation of veratryl compounds were clearly enhanced. In all cultures, the increases in lignin peroxidase titers were directly related to veratryl alcohol accumulation. Evolution of {sup 14}CO{sub 2} from 3-O{sup 14}CH{sub 3}-and 4-O{sup 14}CH{sub 3}-labeled veratric acids showed that the position of the methoxyl substituent in the aromatic ring only slightly affected demeth(ox)ylation activity. In both cases, more than 60% of the total {sup 14}C was converted to {sup 14}CO{sub 2} under air in 4 weeks, and oxygen flux increased the degradation rate of the {sup 14}C-labeled veratric acids just as it did with unlabeled cultures.},
doi = {},
url = {https://www.osti.gov/biblio/6143172}, journal = {Applied and Environmental Microbiology; (USA)},
issn = {0099-2240},
number = ,
volume = 56:9,
place = {United States},
year = {1990},
month = {9}
}