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Title: Reduced growth factor requirement of keloid-derived fibroblasts may account for tumor growth

Abstract

Keloids are benign dermal tumors that form during an abnormal wound-healing process is genetically susceptible individuals. Although growth of normal and keloid cells did not differ in medium containing 10% (vol/vol) fetal bovine serum, keloid culture grew to significantly higher densities than normal cells in medium containing 5% (vol/vol) fetal bovine serum, keloid cultures grew to significantly higher densities than normal cells in medium containing 5% (vol/vol) plasma or 1% fetal bovine serum. Conditioned medium from keloid cultures did not stimulate growth of normal cells in plasma nor did it contain detectable platelet-derived growth factor or epidermal growth factor. Keloid fibroblasts responded differently than normal adult fibroblasts to transforming growth factor ..beta... Whereas transforming growth factor ..beta.. reduced growth stimulation by epidermal growth factor in cells from normal adult skin or scars, it enhanced the activity of epidermal growth factor in cells from normal adult skin or scars, it enhanced the activity of epidermal growth factor in cells from keloids. Normal and keloid fibroblasts also responded differently to hydrocortisone: growth was stimulated in normal adult cells and unaffected or inhibited in keloid cells. Fetal fibroblasts resembled keloid cells in their ability to grow in plasma and in their response tomore » hydrocortisone. The ability of keloid fibroblasts to grow to higher cell densities in low-serum medium than cells from normal adult skin or from normal early or mature scars suggests that a reduced dependence on serum growth factors may account for their prolonged growth in vivo. Similarities between keloid and fetal cells suggest that keloids may result from the untimely expression of growth-control mechanism that is developmentally regulated.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Meharry Medical College, Nashville, TN (USA)
OSTI Identifier:
6142630
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 85:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; FIBROBLASTS; CELL PROLIFERATION; GROWTH FACTORS; BIOCHEMISTRY; WOUNDS; HEALING; BLOOD SERUM; CYTOLOGICAL TECHNIQUES; DOSE-RESPONSE RELATIONSHIPS; HYDROCORTISONE; NEOPLASMS; PATHOLOGICAL CHANGES; SKIN; THYMIDINE; TRITIUM COMPOUNDS; ADRENAL HORMONES; ANIMAL CELLS; AZINES; BIOLOGICAL RECOVERY; BODY; CHEMISTRY; CONNECTIVE TISSUE CELLS; CORTICOSTEROIDS; DISEASES; GLUCOCORTICOIDS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INJURIES; KETONES; LABELLED COMPOUNDS; MITOGENS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PREGNANES; PROTEINS; PYRIMIDINES; RECOVERY; RIBOSIDES; SOMATIC CELLS; STEROIDS; 550301* - Cytology- Tracer Techniques

Citation Formats

Russell, S.B., Trupin, K.M., Rodriguez-Eaton, S., Russell, J.D., and Trupin, J.S. Reduced growth factor requirement of keloid-derived fibroblasts may account for tumor growth. United States: N. p., 1988. Web. doi:10.1073/pnas.85.2.587.
Russell, S.B., Trupin, K.M., Rodriguez-Eaton, S., Russell, J.D., & Trupin, J.S. Reduced growth factor requirement of keloid-derived fibroblasts may account for tumor growth. United States. doi:10.1073/pnas.85.2.587.
Russell, S.B., Trupin, K.M., Rodriguez-Eaton, S., Russell, J.D., and Trupin, J.S. Fri . "Reduced growth factor requirement of keloid-derived fibroblasts may account for tumor growth". United States. doi:10.1073/pnas.85.2.587.
@article{osti_6142630,
title = {Reduced growth factor requirement of keloid-derived fibroblasts may account for tumor growth},
author = {Russell, S.B. and Trupin, K.M. and Rodriguez-Eaton, S. and Russell, J.D. and Trupin, J.S.},
abstractNote = {Keloids are benign dermal tumors that form during an abnormal wound-healing process is genetically susceptible individuals. Although growth of normal and keloid cells did not differ in medium containing 10% (vol/vol) fetal bovine serum, keloid culture grew to significantly higher densities than normal cells in medium containing 5% (vol/vol) fetal bovine serum, keloid cultures grew to significantly higher densities than normal cells in medium containing 5% (vol/vol) plasma or 1% fetal bovine serum. Conditioned medium from keloid cultures did not stimulate growth of normal cells in plasma nor did it contain detectable platelet-derived growth factor or epidermal growth factor. Keloid fibroblasts responded differently than normal adult fibroblasts to transforming growth factor ..beta... Whereas transforming growth factor ..beta.. reduced growth stimulation by epidermal growth factor in cells from normal adult skin or scars, it enhanced the activity of epidermal growth factor in cells from normal adult skin or scars, it enhanced the activity of epidermal growth factor in cells from keloids. Normal and keloid fibroblasts also responded differently to hydrocortisone: growth was stimulated in normal adult cells and unaffected or inhibited in keloid cells. Fetal fibroblasts resembled keloid cells in their ability to grow in plasma and in their response to hydrocortisone. The ability of keloid fibroblasts to grow to higher cell densities in low-serum medium than cells from normal adult skin or from normal early or mature scars suggests that a reduced dependence on serum growth factors may account for their prolonged growth in vivo. Similarities between keloid and fetal cells suggest that keloids may result from the untimely expression of growth-control mechanism that is developmentally regulated.},
doi = {10.1073/pnas.85.2.587},
journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 85:2,
place = {United States},
year = {1988},
month = {1}
}