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Title: Qualitative analysis of mycotoxins using micellar electrokinetic capillary chromatography

Abstract

Naturally occurring mycotoxins are separated using micellar electrokinetic capillary chromatography. Trends in the retention of these toxins, resulting from changes in mobile-phase composition and pH, are reported and presented as a means of alleviating coelution problems. Two sets of mobile-phase conditions are determined that provide unique separation selectivity. The facile manner by which mobile-phase conditions can be altered, without changes in instrumental configuration, allowed the acquisition of two distinctive, fully resolved chromatograms of 10 mycotoxins in a period of approximately 45 min. By adjusting retention times, using indigenous or added components in mycotoxin samples as normalization standards, it is possible to obtain coefficients of variation in retention time that average less than 1%. The qualitative capabilities of this methodology are evaluated by separating randomly generated mycotoxin-interferent mixtures. In this study, the utilization of normalized retention times applied to separations obtained with two sets of mobile-phase conditions permitted the identification of all the mycotoxins in five unknown samples without any misidentifications. 24 refs., 3 figs., 2 tabs.

Authors:
;  [1]
  1. (Univ. of Tennessee, Knoxville (United States))
Publication Date:
OSTI Identifier:
6139265
DOE Contract Number:  
FG05-86ER13613
Resource Type:
Journal Article
Journal Name:
Analytical Chemistry (Washington); (United States)
Additional Journal Information:
Journal Volume: 65:9; Journal ID: ISSN 0003-2700
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; TOXINS; QUALITATIVE CHEMICAL ANALYSIS; CAPILLARIES; CHROMATOGRAPHY; ELECTRODYNAMICS; EXPERIMENTAL DATA; FUNGI; METABOLITES; MICELLAR SYSTEMS; ANTIGENS; BLOOD VESSELS; BODY; CARDIOVASCULAR SYSTEM; CHEMICAL ANALYSIS; DATA; HAZARDOUS MATERIALS; INFORMATION; MATERIALS; NUMERICAL DATA; ORGANS; PLANTS; SEPARATION PROCESSES; TOXIC MATERIALS; 560300* - Chemicals Metabolism & Toxicology; 400100 - Analytical & Separations Chemistry

Citation Formats

Holland, R.D., and Sepaniak, M.J. Qualitative analysis of mycotoxins using micellar electrokinetic capillary chromatography. United States: N. p., 1993. Web. doi:10.1021/ac00057a007.
Holland, R.D., & Sepaniak, M.J. Qualitative analysis of mycotoxins using micellar electrokinetic capillary chromatography. United States. doi:10.1021/ac00057a007.
Holland, R.D., and Sepaniak, M.J. Sat . "Qualitative analysis of mycotoxins using micellar electrokinetic capillary chromatography". United States. doi:10.1021/ac00057a007.
@article{osti_6139265,
title = {Qualitative analysis of mycotoxins using micellar electrokinetic capillary chromatography},
author = {Holland, R.D. and Sepaniak, M.J.},
abstractNote = {Naturally occurring mycotoxins are separated using micellar electrokinetic capillary chromatography. Trends in the retention of these toxins, resulting from changes in mobile-phase composition and pH, are reported and presented as a means of alleviating coelution problems. Two sets of mobile-phase conditions are determined that provide unique separation selectivity. The facile manner by which mobile-phase conditions can be altered, without changes in instrumental configuration, allowed the acquisition of two distinctive, fully resolved chromatograms of 10 mycotoxins in a period of approximately 45 min. By adjusting retention times, using indigenous or added components in mycotoxin samples as normalization standards, it is possible to obtain coefficients of variation in retention time that average less than 1%. The qualitative capabilities of this methodology are evaluated by separating randomly generated mycotoxin-interferent mixtures. In this study, the utilization of normalized retention times applied to separations obtained with two sets of mobile-phase conditions permitted the identification of all the mycotoxins in five unknown samples without any misidentifications. 24 refs., 3 figs., 2 tabs.},
doi = {10.1021/ac00057a007},
journal = {Analytical Chemistry (Washington); (United States)},
issn = {0003-2700},
number = ,
volume = 65:9,
place = {United States},
year = {1993},
month = {5}
}