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Title: Potentially lethal damage versus sublethal damage: independent repair processes in actively growing Chinese hamster cells. [Effects of osmotic pressure differences due to NaCl concentrations in buffer solutions on sensitivity of cells to x rays]

Abstract

Actively growing V79 Chinese hamster cells, treated with anisotonic phosphate-buffered saline (PBS) after x irradiation, are more sensitive than cells treated with isotonic PBS or cells promptly incubated with complete medium immediately after irradiation. The sensitization of irradiated cells results from hypotonic as well as hypertonic NaCl concentrations in PBS, is strongly dependent on both temperature and time, and is mainly due to an increase in the final slope of the single-dose survival curve. After two x-ray dose fractions, the net response of cells sensitized after each fraction by anisotonic post-treatment is similar to that obtained for isotonically treated cells and indicates that sublethal damage repair is not influenced by the enhanced expression of lethal damage. Independence of the repair of damage which is potentially lethal from the repair of damage which is sublethal is further suggested by the more rapid rate of the former compared to that of the latter. The proposal is advanced that the enhanced expression of damage which, after x irradiation, can be shown to be potentially lethal results from a destabilization of the structural relationship between DNA and the nuclear envelope, and/or DNA and the nuclear protein matrix, as a consequence of osmotic changes producedmore » by anisotonic treatment.« less

Authors:
;
Publication Date:
Research Org.:
Argonne National Lab., IL
OSTI Identifier:
6138109
Resource Type:
Journal Article
Journal Name:
Radiat. Res.; (United States)
Additional Journal Information:
Journal Volume: 77:2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ANIMAL CELLS; RADIOSENSITIVITY; DNA; BIOLOGICAL REPAIR; HYPERTONIC SOLUTIONS; RADIOSENSITIVITY EFFECTS; OSMOSIS; TEMPERATURE DEPENDENCE; TIME DEPENDENCE; ACUTE IRRADIATION; AGE DEPENDENCE; ANOXIA; BIOLOGICAL RADIATION EFFECTS; BUFFERS; CELL CULTURES; COMPARATIVE EVALUATIONS; EXPERIMENTAL DATA; FRACTIONATED IRRADIATION; GRAPHS; ISOLATED VALUES; LETHAL IRRADIATION; SODIUM CHLORIDES; SOLUTIONS; SUBLETHAL IRRADIATION; ULTRAVIOLET RADIATION; X RADIATION; ACUTE EXPOSURE; ALKALI METAL COMPOUNDS; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; CHLORIDES; CHLORINE COMPOUNDS; DATA; DATA FORMS; DISPERSIONS; ELECTROMAGNETIC RADIATION; HALIDES; HALOGEN COMPOUNDS; INFORMATION; IONIZING RADIATIONS; IRRADIATION; MIXTURES; NUCLEIC ACIDS; NUMERICAL DATA; ORGANIC COMPOUNDS; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; SODIUM COMPOUNDS; 560121* - Radiation Effects on Cells- External Source- (-1987)

Citation Formats

Utsumi, H., and Elkind, M.M. Potentially lethal damage versus sublethal damage: independent repair processes in actively growing Chinese hamster cells. [Effects of osmotic pressure differences due to NaCl concentrations in buffer solutions on sensitivity of cells to x rays]. United States: N. p., 1979. Web. doi:10.2307/3575146.
Utsumi, H., & Elkind, M.M. Potentially lethal damage versus sublethal damage: independent repair processes in actively growing Chinese hamster cells. [Effects of osmotic pressure differences due to NaCl concentrations in buffer solutions on sensitivity of cells to x rays]. United States. doi:10.2307/3575146.
Utsumi, H., and Elkind, M.M. Thu . "Potentially lethal damage versus sublethal damage: independent repair processes in actively growing Chinese hamster cells. [Effects of osmotic pressure differences due to NaCl concentrations in buffer solutions on sensitivity of cells to x rays]". United States. doi:10.2307/3575146.
@article{osti_6138109,
title = {Potentially lethal damage versus sublethal damage: independent repair processes in actively growing Chinese hamster cells. [Effects of osmotic pressure differences due to NaCl concentrations in buffer solutions on sensitivity of cells to x rays]},
author = {Utsumi, H. and Elkind, M.M.},
abstractNote = {Actively growing V79 Chinese hamster cells, treated with anisotonic phosphate-buffered saline (PBS) after x irradiation, are more sensitive than cells treated with isotonic PBS or cells promptly incubated with complete medium immediately after irradiation. The sensitization of irradiated cells results from hypotonic as well as hypertonic NaCl concentrations in PBS, is strongly dependent on both temperature and time, and is mainly due to an increase in the final slope of the single-dose survival curve. After two x-ray dose fractions, the net response of cells sensitized after each fraction by anisotonic post-treatment is similar to that obtained for isotonically treated cells and indicates that sublethal damage repair is not influenced by the enhanced expression of lethal damage. Independence of the repair of damage which is potentially lethal from the repair of damage which is sublethal is further suggested by the more rapid rate of the former compared to that of the latter. The proposal is advanced that the enhanced expression of damage which, after x irradiation, can be shown to be potentially lethal results from a destabilization of the structural relationship between DNA and the nuclear envelope, and/or DNA and the nuclear protein matrix, as a consequence of osmotic changes produced by anisotonic treatment.},
doi = {10.2307/3575146},
journal = {Radiat. Res.; (United States)},
number = ,
volume = 77:2,
place = {United States},
year = {1979},
month = {2}
}