Cytotoxic effect of vanadium and oil-fired fly ash on hamster tracheal epithelium

Journal Article · Wed Aug 01 00:00:00 EDT 1984 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/0013-9351(84)90105-1· OSTI ID:6133894

Schiff, L J; Graham, J A

Hamster tracheal organ cultures were used to study the in vitro effects of vanadium and oil-fired fly ash on mucociliary respiratory epithelium. Two vanadium compounds, VOSO/sub 4/ and V/sub 2/O/sub 5/, and fly ash from an oil-fueled power plant were dissolved or suspended in culture medium over a range of concentrations and epithelia were exposed for 1 hr/day, for 9 consecutive days. At intervals during this period, alterations in cilia-beating frequency, cytology, and histology were documented by light microscopy. Explants treated with VOSO/sub 4/ either decreased ciliary activity or produced ciliostasis depending upon the concentration and length of exposure. Early morphological alterations consisted of vacuolization of both nuclei and cytoplasm. After multiple exposures, cytology of VOSO/sub 4/-treated respiratory mucosa was markedly affected. Similar changes were observed in cultures exposed to V/sub 2/O/sub 5/; however, the cytotoxicity appeared earlier and was more pronounced. Fly ash-treated explants produced similar biological effects when compared to both vanadium compounds. Thus, the data indicate that the extent of vanadium toxicity depends, at least in part, on the vanadium content of the compound tested, and that exposure to this metal and vanadium-rich fly ash can inhibit normal mucociliary function, a vital clearance mechanism in the respiratory tract.

• Research Organization: IIT Research Inst., Chicago, IL
• OSTI ID: 6133894
• Journal Information: Environ. Res.; (United States), Vol. 34:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
EPITHELIUM
MORPHOLOGICAL CHANGES
FLY ASH
TOXICITY
VANADIUM
CLEARANCE
COMBUSTION PRODUCTS
CYTOLOGY
EXPERIMENTAL DATA
FOSSIL-FUEL POWER PLANTS
HAMSTERS
IN VITRO
INHIBITION
MUCOUS MEMBRANES
PETROLEUM
RESPIRATORY SYSTEM
AEROSOL WASTES
ANIMAL TISSUES
ANIMALS
ASHES
BIOLOGY
BODY
DATA
ELEMENTS
ENERGY SOURCES
FOSSIL FUELS
FUELS
INFORMATION
MAMMALS
MEMBRANES
METALS
NUMERICAL DATA
POWER PLANTS
RESIDUES
RODENTS
THERMAL POWER PLANTS
TISSUES
TRANSITION ELEMENTS
VERTEBRATES
WASTES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)